Earticle

초록

영어

N-glycosylation is one of the major post-translational protein modification, which alters physicochemical properties of the protein, affecting the folding, distribution, stability and thus biological function and efficiency of protein. Although the earlier steps in the N-glycosylation pathway leading to the formation of oligomannosidic structures are conserved in plants and mammals, the later steps in the formation of complex N-glycans are quite different from each other. In particular, plant type complex N-glycans are distinctive from those found in mammalian because they contain ß1,2-xylose and core α1,3-fucose residues attached to the pentasaccharide (Man3GlcNAc2) core structure but no sialic acid residues. The presence of ß1,2-xylose and core α1,3-fucose residues on plant type complex N-glycans has long been an irritating limitation in the use of plant-made pharmaceuticals (PMPs) in human therapy, as these N-glycan epitopes are potentially immunogenic in mammals. In this study, to remove the plant specific sugar residues and humanize the N-glycosylation in plant, we isolated mutants of the corresponding plant specific glycosyltransferase genes and used for multiple-mutants construction. The resulting mutants will be transformed by human glycosyltransferases genes to accomplish humanized N-glycosylation in plant.

저자

• Ki Seong Ko [ Division of Applied Life Science (BK21) and PMBBRC, Gyeongsang National University ]
• Wahyu Indra Fanata [ Division of Applied Life Science (BK21) and PMBBRC, Gyeongsang National University ]
• Bo Hwa Son [ Division of Applied Life Science (BK21) and PMBBRC, Gyeongsang National University ]
• Jae Yong Yoo [ Division of Applied Life Science (BK21) and PMBBRC, Gyeongsang National University ]
• Rikno Harmoko [ Division of Applied Life Science (BK21) and PMBBRC, Gyeongsang National University ]
• Nirmal Kumar Ramasamy [ Division of Applied Life Science (BK21) and PMBBRC, Gyeongsang National University ]
• Kyung Hwa Kim [ Division of Applied Life Science (BK21) and PMBBRC, Gyeongsang National University ]
• Thiyagarajan Thulasinathan [ Division of Applied Life Science (BK21) and PMBBRC, Gyeongsang National University ]
• Sang Yeol Lee [ Division of Applied Life Science (BK21) and PMBBRC, Gyeongsang National University ]
• Kyun Oh Lee [ Division of Applied Life Science (BK21) and PMBBRC, Gyeongsang National University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국당과학회 학술대회

• 간기

  연간

• 수록기간

  2006~2022

• 십진분류

  KDC 517 DDC 614