Earticle

현재 위치 Home

PP-36

BLT1-mediated O-glycosylation is involved in CREB and NF-, B –dependent IL-8 production in human mast cell induced by protozoan parasite Trichomonas vaginalis-secretory products

첫 페이지 보기
  • 발행기관
    한국당과학회 바로가기
  • 간행물
    한국당과학회 학술대회 바로가기
  • 통권
    ACGG 2012 Conference (2012.10)바로가기
  • 페이지
    pp.60-61
  • 저자
    Young Hee Nam, Arim Min, Young-Ah Lee, Kyoung-Ju Song, Myeong Heon Shin
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A192841

※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.

원문정보

초록

영어
Trichomonas vaginalis is a flagellated protozoan parasite causing vaginal trichomoniasis in women, and can secrete chemotactic lipid mediator LTB4 in their secretory products. However, signaling mechanisms of tissue inflammatory responses by infection with T. vaginalis are not fully understood. Recently, we have recently demonstrated that human mast cells and neutrophils are activated to produce chemokine IL-8 via LTB4 receptor BLT1 in response to T. vaginalis-derived secretory products (TvSP). Here, we report that O-glycosylation is important in BLT1-mediated IL-8 secretion in human mast cells (HMC-1 cell line) induced by TvSP. Incubation of HMC-1 cells with TvSP resulted in marked increase of O-GlcNacylated proteins and up-regulated IL-8 protein secretion. TvSP-induced IL-8 production and O-glycosylation in HMC-1 cells was inhibited by pretreatment with OGT inhibitor or OGT siRNA. Pretreatment of HMC-1 cells with BLT1 antagonist or BLT1 siRNA strongly abolished the stimulatory effects of TvSP on O-GlcNacylation and IL-8 production. Moreover, TvSP-induced phosphorylation of transcription factors NF-B and CREB for IL-8 production was reduced by pretreatment of HMC-1 cells with OGT inhibitor or OGT siRNA. These results suggest that BLT1-mediated O-glycosylation may play an important role in activation of transcription factors CREB and NF-B for IL-8 production in mast cells stimulated with TvSP, which can contribute to mast cell-mediated tissue inflammatory responses in T. vaginalis-infected lesion during human trichomoniasis.

저자

  • Young Hee Nam [ Department of Environmental Medical Biology and Institute of Tropical Medicine ]
  • Arim Min [ Department of Environmental Medical Biology and Institute of Tropical Medicine, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, Korea ]
  • Young-Ah Lee [ Department of Environmental Medical Biology and Institute of Tropical Medicine ]
  • Kyoung-Ju Song [ Department of Environmental Medical Biology and Institute of Tropical Medicine ]
  • Myeong Heon Shin [ Department of Environmental Medical Biology and Institute of Tropical Medicine, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국당과학회 [Korean Society for Glycoscience]
  • 설립연도
    2006
  • 분야
    의약학>약학
  • 소개
    본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.

간행물

  • 간행물명
    한국당과학회 학술대회
  • 간기
    연간
  • 수록기간
    2006~2022
  • 십진분류
    KDC 517 DDC 614

이 권호 내 다른 논문 / 한국당과학회 학술대회 ACGG 2012 Conference

    피인용수 : 0건 (자료제공 : 네이버학술정보)

    함께 이용한 논문 이 논문을 다운로드한 분들이 이용한 다른 논문입니다.

      출처 : 네이버학술정보

        0개의 논문이 장바구니에 담겼습니다.

        페이지 저장
        소속기관 조회
        이용자님의 소속기관(단체)이 서비스에 가입되어 있는지 확인해 보십시오.
        기관회원에 소속되어 있는 이용자는 원문을 무료로 이용할 수 있습니다.