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PP-20, Session 7: Glycobiotechnology II, Chairperson: Eiji Miyoshi, Chaisiri Wongkham

Expression patterns of gangliosides in lipopolysaccharid-stimulated normal PK-15 cells and gangliosides knock-down PK15 cells

첫 페이지 보기
  • 발행기관
    한국당과학회 바로가기
  • 간행물
    한국당과학회 학술대회 바로가기
  • 통권
    ACGG 2012 Conference (2012.10)바로가기
  • 페이지
    pp.50-50
  • 저자
    Ju-Taek Lee, Jae-Sung Ryu, Ghislain Moussavou, Malg-Um Lim, Hyun-Ki Min, Yoon-Ju Na, Su-Bin Lee, In-Sun Kim, Ju-Hyoung Lee, Ji-Su Kim, Sun-Uk Kim, Kyu-Tae Chang, Young-Kug Choo
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A192816

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원문정보

초록

영어
Glycosphingolipids including gangliosides play important regulatory roles in cell prolifera-tion and differentiation. UDP-glucose:ceramide glucosyltransferase(Ugcg) catalyze the initial step in glycosphingolipids biosynthesis pathway. In this study, Ugcg expression was reduced to approximately 80% by short hairpin RNAs(shRNAs) to evaluate the roles of glycosphingolipids in proliferation and gangliosdies differentiation of PK15. HPTLC/immunofluorescence analyses of shRNA transfected PK15 revealed that treatment with Ugcg-shRNA decreased expression of major gangliosides, GM1 and GD1b. Furthermore, MTT and western blot/immunofluorescence analyses demonstreated that inhibition of the Ugcg expression in PK15 resulted in decrease of cell proliferation. In addition to we showed that inflammatory respon-ses in PK15 and gangliosides knock-down PK15 cells stimulated with Escherichia coli lipopolysaccharide(LPS). The gangliosides GM3, GM2, GD3, GD1a and GD1b were detected in normal PK15 cell. However almost gangliosides undetected in gangliosides knock-down PK15 cell. In addition we found that the expression levels of TNF-α, indu-cible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) and we measured the production of NO. The expression of inflammatory proteins, TNF- α, iNOS and COX-2 the elevated concentration in gangliosides knock-down PK15 cell. These results suggest that gangliosides interact with components of the proinflammatory response pathway and might, therefore, be relevant for designing future therapeutic strategies intended to prolong xenograft survival.

저자

  • Ju-Taek Lee [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Jae-Sung Ryu [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Ghislain Moussavou [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Malg-Um Lim [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Hyun-Ki Min [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Yoon-Ju Na [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Su-Bin Lee [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • In-Sun Kim [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Ju-Hyoung Lee [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Ji-Su Kim [ National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB) ]
  • Sun-Uk Kim [ National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB) ]
  • Kyu-Tae Chang [ National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB) ]
  • Young-Kug Choo [ Department of Biological Science, College of Natural Sciences, Wonkwang University ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국당과학회 [Korean Society for Glycoscience]
  • 설립연도
    2006
  • 분야
    의약학>약학
  • 소개
    본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.

간행물

  • 간행물명
    한국당과학회 학술대회
  • 간기
    연간
  • 수록기간
    2006~2022
  • 십진분류
    KDC 517 DDC 614

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