PP-15, Session 7: Glycobiotechnology II, Chairperson: Eiji Miyoshi, Chaisiri Wongkham
Using High Performance Liquid Chromatography on a Microfluidic Chip and Time-of-Flight Mass Spectrometryfor Identification of Native N-linked Glycan Structures from Monoclonal Antibody
Glycan play key roles in protein folding, cell-cell recognition, cancer metastasis, and the immune system. In cell, glycosylation comprise that many kinds of glycan structures associated with a specific glycoprotein have different functions. So, the biological meaning of glycosylation has made them a prime target for discovering biomarker in diseases. Identifying the numerous glycan, we have used new method to assess the diversity of the N-linked oligosaccharides without derivatization has been developed using on-line nano-liquid chromatography (nanoLC) and high resolution time-of-flight mass spectrometry. In three times MS/MS results, chips offered good sensitivity and reproducibility in separating a intact protein. In tandem mass spectra, glycan fragment ions were identified as N-glycan sequences for mining glycoproteome. As a result, we have identified several N-glycan structures from monoclonal antibody.
저자
Jong-Moon Park [ Lee Gil Ya Cancer and Diabetes Institute, Gachon University ]
Na-Young Han [ Lee Gil Ya Cancer and Diabetes Institute, Gachon University ]
Hookeun Lee [ Lee Gil Ya Cancer and Diabetes Institute, Gachon University, College of Pharmacy, Gachon University ]
본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.