Earticle

현재 위치 Home

PP-1, Session 2: Glycobiology II, Chairperson: Chun-Hung Lin, Sopit Wongkham

Site-specific N-glycosylation identification of recombinant human lectin-like oxidized low density lipoprotein receptor-1 (LOX-1)

첫 페이지 보기
  • 발행기관
    한국당과학회 바로가기
  • 간행물
    한국당과학회 학술대회 바로가기
  • 통권
    ACGG 2012 Conference (2012.10)바로가기
  • 페이지
    pp.37-37
  • 저자
    Yifan Qian, Xingwang Zhang, Lei Zhou, Shifang Ren, Jianxin Gu
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A192789

※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.

원문정보

초록

영어
Human LOX-1/OLR 1 plays a key role in atherogenesis and endothelial dysfunction. The N-glycosylation of LOX-1 has been shown to affect its biological functions in vivo and modulate the pathogenesis of atherosclerosis. However, the N-glycosylation pattern of LOX-1 has not been described yet. The present study was aimed at elucidating the N-glycosylation of recombinanthuman LOX-1 with regard to N-glycan profile and N-glycosylation sites. Here, an approach using nonspecific protease (Pronase E) digestion followed by MALDI-QIT-TOF MS and multistage MS (MS3) analysis is explored to obtain site-specificN- glycosylation information of recombinanthuman LOX-1, in combination with glycan structure confirmation through characterizing released glycans using tandem MS. The results reveal that N-glycans structures as well as their corresponding attached site of LOX-1 can be identified simultaneously by direct MS analysis of glycopeptides from non-specific protease digestion. With this approach, one potential glycosylation site of recombinanthuman LOX-1 on Asn139 is readily identified and found to carry heterogeneous complex type N-glycans. In addition, manual annotation of multistage MS data utilizing diagnostic ions, which were found to be particularly useful in defining the structure of glycopeptides and glycans was addressed for proper spectra interpretation. The findings described herein will shed new light on further research of the structure-function relationships of LOX-1 N-glycan.

저자

  • Yifan Qian [ Key Laboratory of Glycoconjugate Research Ministry of Public Health; Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan University, Shanghai, P.R.China, 200032 ]
  • Xingwang Zhang [ Key Laboratory of Glycoconjugate Research Ministry of Public Health; Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan University, Shanghai, P.R.China, 200032 ]
  • Lei Zhou [ Key Laboratory of Glycoconjugate Research Ministry of Public Health; Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan University, Shanghai, P.R.China, 200032 ]
  • Shifang Ren [ Key Laboratory of Glycoconjugate Research Ministry of Public Health; Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan University, Shanghai, P.R.China, 200032 ]
  • Jianxin Gu [ Key Laboratory of Glycoconjugate Research Ministry of Public Health; Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan University, Shanghai, P.R.China, 200032 ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국당과학회 [Korean Society for Glycoscience]
  • 설립연도
    2006
  • 분야
    의약학>약학
  • 소개
    본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.

간행물

  • 간행물명
    한국당과학회 학술대회
  • 간기
    연간
  • 수록기간
    2006~2022
  • 십진분류
    KDC 517 DDC 614

이 권호 내 다른 논문 / 한국당과학회 학술대회 ACGG 2012 Conference

    피인용수 : 0건 (자료제공 : 네이버학술정보)

    함께 이용한 논문 이 논문을 다운로드한 분들이 이용한 다른 논문입니다.

      출처 : 네이버학술정보

        0개의 논문이 장바구니에 담겼습니다.

        페이지 저장
        소속기관 조회
        이용자님의 소속기관(단체)이 서비스에 가입되어 있는지 확인해 보십시오.
        기관회원에 소속되어 있는 이용자는 원문을 무료로 이용할 수 있습니다.