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원문정보
초록
영어
Embryonic stem cell classically cultured on feeder layer with FBS contained ES medium. Feeder-free mouse ES cell culture systems are essential to avoid the possible contamination of nonES cells. First we determined the difference between ES cell and MEF by Oct4 population. We demonstrate to culture and to induce differentiation on feeder free condition using a commercially available mouse ES cell lines.
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS Cell Lines Feeder Preparation and Embryonic Stem (ES) Cell Culture Feeder Free Culture of Embryonic Stem (ES) Cell Induce Differentiation by Withdrawal of LIF RESULTS Phenotype of ES Cell and Feeder The ES Cell Culture on the Feeder Free Condition The ES Cell Induce Differentiation on the Feeder FreeCondition DISCUSSION REFERENCES
키워드
ES cellsDifferentiationFeeder free culture
저자
Myoung Ok Kim [ School of Ecology and Environmental Sciences, School of Life Sciences and Biotechnology, Kyungpook National University, Daegu 702-701, Korea ]
Zae Young Ryoo [ School of Ecology and Environmental Sciences, School of Life Sciences and Biotechnology, Kyungpook National University, Daegu 702-701, Korea ]
Corresponding author