Embryonic Stem Cell-Preconditioned Microenvironment Effects on Epidermoid Carcinoma
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- 발행기관
- 한국동물생명공학회(구 한국동물번식학회) 바로가기
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- 간행물
- Reproductive & developmental biology 바로가기
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- 통권
- Volume 36 No 4 (2012.12)바로가기
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- 페이지
- pp.275-281
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- 저자
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- 언어
- 영어(ENG)
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- URL
- https://www.earticle.net/Article/A192768
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원문정보
초록
- 영어
- Embryonic stem cell-preconditioned microenvironment is important for cancer cells properitities by change cell morphology and proliferation. This microenvironment induces cancer cell reprogramming and results in a change in cancer cell properties such as differentiation and migration. The cancer microenvironment affects cancer cell proliferation and growth. However, the mechanism has not been clarified yet. Using the ES-preconditioned 3-D microenvironment model, we provide evidence showing that the ES microenvironment inhibits proliferation and reduces oncogenic gene expression. But ES microenvironment has no effect on telomerase activity, cell viability, cellular senescence, and methylation on Oct4 promoter region. Furthermore, methylation of Nanog was increase on ES-preconditioned microenvironment and supports results that no difference on RNA expression levels. Taken together, these results demonstrated that in the ES-preconditioned 3-D microenvironment is a crucial role for cancer cell proliferation not senescence.
목차
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Cell Culture
Feeder Preparation and Embryonic Stem (ES) Cell Culture
Preparation of 3D-Conditioned Matrix and Reprogramming Induction
Cell Viability Test
Bisulfite Sequencing
RNA Extraction and Real Time RT-PCR
3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfonyl)-2H-tetrazolium Assay
Cell Cycle Analysis
Telomerase Activity Assay
Cellular Aging Assay
RESULTS
The ES-Preconditioned Microenvironment Induces MorphologicalChanges
The ES-Preconditioned Microenvironment Induces Lossof Cancer Cell Properities
The ES-Preconditioned Microenvironment Not Induces Cellular Senescence
ES-Preconditioned Microenvironment Suppress OncogenicGene Expression
DISCUSSION
Acknowledgements
REFERENCES
키워드
ES cells Microenvironment 3D culture Reprogramming저자
간행물 정보
발행기관
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- 발행기관명
- 한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
- 설립연도
- 1976
- 분야
- 농수해양>축산학
- 소개
- 동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.
간행물
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- 간행물명
- Reproductive & developmental biology
- 간기
- 계간
- pISSN
- 1738-2432
- eISSN
- 2288-0151
- 수록기간
- 1977~2018
- 십진분류
- KDC 527 DDC 636
이 권호 내 다른 논문 / Reproductive & developmental biology Volume 36 No 4
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