Earticle

초록

영어

The present study was to assess the in vitro viability and sexing rate of bovine embryos. Blastocysts were harvested on day 7～9 day after insemination(in vitro and in vivo), and the sex of the embryos was examined using the LAMP method. Embryo cell biopsy was carried out in a 80 μl drop Ca2+, Mg2+ free D-PBS and, biopsied embryos viability were evaluated after more 12 h culture in IVMD culture medium. The formation of recovered embryo to expanded and hatching stages had ensued in higher of sexed embryo in vivo than in vitro (100% vs. 89%, p<0.05), and in vitro, the rates of degeneration after sexing were significantly (p<0.05) higher in vitro than in vivo(11% vs. 0.0%). The rates of the predicted sex were female 61% vs. 56%, and male 39% vs. 44% in vivo and in vitro, respectively. The rates of survival following different biopsy methods were seen between punching and bisection method in vivo and in vitro (100% vs. 89% and 100% vs, 78% respectively). Biopsy method by punching was significantly (p<0.05) higher than bisection between produced embryos in vivo and in vitro. The present data indicate that with microblade after punching for embryo sexing results in high incidence of survivability on development after embryo biopsy. It is also suggested that LAMP-based embryo sexing suitable for field applications.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  In Vitro Embryo Production
  In Vivo Embryo Production
  Embryo Biopsy
  LAMP Analysis
  Experimental Design
  Statistical Analysis
 RESULTS
 DISCUSSION
 REFERENCES

키워드

저자

• Sang-Rae Cho [ Subtropical Animal Experimental Station, National Livestock Institute, Rural Development Administration, Jeju, 690-150, Korea ] Corresponding author
• Chang-Young Choe [ Dairy Science Division, National Livestock Institute, Rural Development Administration, Cheonan, 331-801, Korea ]
• Jun-Kyu Son [ Dairy Science Division, National Livestock Institute, Rural Development Administration, Cheonan, 331-801, Korea ]
• In-Cheol Cho [ Subtropical Animal Experimental Station, National Livestock Institute, Rural Development Administration, Jeju, 690-150, Korea ]
• Jae-Gyu Yoo [ Animal Biotechnology Division, National Livestock Institute, Rural Development Administration, Suwon 441-706, Korea ]
• Hyung-Jong Kim [ Planning & Coordination, National Livestock Institute, Rural Development Administration, Suwon 441-706, Korea ]
• Yeong-Gyu Ko [ Division, Animal Genetic Resources Station, National Livestock Institute, Rural Development Administration, Namwon, 590-830, Korea ]
• Nam-Young Kim [ Subtropical Animal Experimental Station, National Livestock Institute, Rural Development Administration, Jeju, 690-150, Korea ]
• Sang-Hyun Han [ Subtropical Animal Experimental Station, National Livestock Institute, Rural Development Administration, Jeju, 690-150, Korea ]
• Yong-Sang Park [ Subtropical Animal Experimental Station, National Livestock Institute, Rural Development Administration, Jeju, 690-150, Korea ]
• Moon-Suck Ko [ Subtropical Animal Experimental Station, National Livestock Institute, Rural Development Administration, Jeju, 690-150, Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636