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Successful In Vitro Development of Preantral Follicles Isolated from Vitrified Mouse Whole Ovaries

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 36 No 4 (2012.12)바로가기
  • 페이지
    pp.255-260
  • 저자
    Dong-Hoon Kim, Jin-Gu No, Jong-Ju Park, Jin-Ki Park, Jae Gyu Yoo
  • 언어
    한국어(KOR)
  • URL
    https://www.earticle.net/Article/A192755

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초록

영어
The purpose of this study was to assess follicular viability and competence through in vitro culture of preantral follicles isolated from vitrified mouse whole ovaries. Mouse preantral follicles were enzymatically isolated from vitrified-warmed and fresh ovaries and cultured for 10 days followed by in vitro oocyte maturation. In vitro matured oocytes were fertilized and cultured to the blastocyst stage. Five minutes pre-exposure to vitrification solution of whole ovaries had significantly higher (p<0.05) oocyte survival and maturation rates than between 10 min exposure groups. Oocyte diameter was significantly smaller (p<0.05) in the 5 and 10 min exposure groups (69.4±2.8 and 67.8±3.1) when compared to that of control group (71.7±2.1). There was no statistical significant difference in blastocyst development rates between vitrification group (8.6%) and the fresh control group (12.0%). The mean number of cells per blastocyst was significantly lower (p<0.05) in the vitrification group (41.9±20.2) than in the fresh control group (55.1±22.5). The results show that mouse oocytes within preantral follicles isolated from the vitrified whole ovaries can achieve full maturation, normal fertilization and embryo development.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Collection of Ovaries
  Vitrification and Thawing
  Preantral Follicle Isolation
  In Vitro Growth (IVG) of Preantral follicles
  In Vitro Maturation (IVM), Fertilization (IVF) and SubsequentDevelopment
  Assessment of Cell Number of Blastocyst
  Statistical Analysis
 RESULTS
  Recovery of Preantral Follicles from Vitrified Ovary
  In Vitro Growth and Maturation of Preantral Follicles Isolated from Vitrified Ovary
  In Vitro Development of Oocytes within Preantral Follicles
 DISSCUSSION
 REFERENCES

키워드

Vitrification Open straw (OS) Mouse ovary Preantral follicle

저자

  • Dong-Hoon Kim [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon 441-706, Korea ] Corresponding author
  • Jin-Gu No [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon 441-706, Korea ]
  • Jong-Ju Park [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon 441-706, Korea ]
  • Jin-Ki Park [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon 441-706, Korea ]
  • Jae Gyu Yoo [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon 441-706, Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Reproductive & developmental biology Volume 36 No 4

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