Earticle

초록

영어

This study aimed at investigating whether a porcine follicular fluid (pFF) supplementation positively affects the characteristics of donor cells and the developmental competence of porcine cloned embryos. Ear fibroblast cells (donor cell) from an Massachusetts General Hospital miniature pig were cultured in different culture methods: (1) Dulbecco's modified Eagle's medium (DMEM)+10% FBS (Control); (2) DMEM+0.5% FBS (SS); and (3) DMEM+10% FBS+10% pFF (pFF) for 72 h. In each conditioned medium, the concentrations of 4 amino acids (Thr, Glu, Pro, and Val) in the pFF group were significantly different from those in the control group (p<0.05 or p<0.01). The proliferation of the cells cultured in the SS group was significantly lower than that of the other treatment groups (p<0.01). The population of apoptotic and necrotic cells in the SS group was significantly higher than that of either the control or the pFF group (p<0.01). The number of embryos that cleaved (p<0.05) and developed into blastocysts (p<0.01) in the SS group was significantly lower than that of either the control or the pFF group. Compared to other groups, the blastocysts produced from the donor cells in the pFF group had higher total cells and lower apoptotic cells (p<0.05). It can be concluded that pFF supplementation in the donor cell culture medium positively affects cell death, cell cycle and quality of the cloned blastocyst.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Preparation of Cumulus-Oocyte Complexes (COCs) and FF
  Isolation and Culture of Miniature Pig Ear Fibroblasts
  Determination of Amino Acid Concentrations
  Cell Proliferation Assay
  Determination of Apoptotic and Necrotic Cell Distribution
  SCNT Procedure
  TUNEL Assay for Apoptotic Cell Death
  Statistical Analysis
 RESULTS
  Measurement of Amino Acid Concentration
  Cell Proliferation Assay
  Determination of Apoptotic and Necrotic Cells
  Developmental Competence of Cloned Embryos
  Apoptotic Cell Death in Cloned Blastocysts
 DISCUSSION
 ACKNOWLEDGEMENTS
 REFERENCES

키워드

저자

• Dae-Jin Kwon [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon 441-706, Korea ]
• Keon Bong Oh [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon 441-706, Korea ]
• Sun A Ock [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon 441-706, Korea ]
• Jeong Woong Lee [ Regenerative Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea ]
• Sung-Soo Lee [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon 441-706, Korea ]
• Jin-Ki Park [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon 441-706, Korea ]
• Won-Kyong Chang [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon 441-706, Korea ]
• Seongsoo Hwang [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon 441-706, Korea ] Corresponding author

참고문헌

발행기관

간행물

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• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636