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Genomic Polymorphism Analysis using Microsatellite Markers in Gyeongju Donggyeong Dogs

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 36 No 4 (2012.12)바로가기
  • 페이지
    pp.243-248
  • 저자
    Seung-Chang Kima, Lee-Kyung Kima, Chang-Min Park, Sun-Ae Park, Yong-Min Cho, Dajeong Lim, Han-Ha Chai, Seung-Hwan Lee, Bong-Hwan Choi, Seog-Kyu Choia, Ji-Woong Lee, Sang-Soo Sun
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A192753

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원문정보

초록

영어
This study was conducted to find a useful marker for gene polymorphism analysis using Microsatellite marker (MS marker) in Gyeongju Donggyeong dog. Twenty three MS marker analyzed the genetic features of DNA using 100 Gyeongju Donggyeong dogs in Gyeongju area. It was performed multiplex PCR with 3 set primer divided 9, 10 and 4 by analysis of conditions among MS markers. The results were calculated heterozygosity, polymorphic information content (PIC), allele frequency and number of allele at each locus using Microsatellite Toolkit software and Cervus 3.0 program. Total 148 alleles were genotyped to determine and average 6.43 alleles was detected. FH3381 had the highest of 15 alleles and FH2834 had the lowest of 2 alleles. Expected heterozygosity had a wide range from 0.282 to 0.876 and had average value of 0.6496. Also, Observed heterozygosity had a more wide range from 0.200 to 0.950 and had average value of 0.6404. PIC had range from 0.262 to 0.859 and average PIC was calculated 0.606. Especially, FH2998 represented the highest rate of observed heterozygosity of 0.950 and FH3381 represented the highest rate of expected heterozygosity of 0.876 and PIC of 0.859. The use of these markers was considered to be useful to study genetic traits of Gyeongju Donggyeong dog.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Animals and Extraction of Genomic DNA
  MS Marker for Allele Analysis
  Multiplex PCR and MS Analysis
  Statistic analysis
 RESULTS AND DISCUSSION
 REFERENCES

키워드

Microsatellite marker Canine Gyeongju Donggyeong dog Allele Heterozygosity

저자

  • Seung-Chang Kima [ Division of Animal Genomics & Bioinformatics, National Institute of Animal Science, RDA, Suwon 441-706, Republic of Korea ]
  • Lee-Kyung Kima [ Division of Animal Genomics & Bioinformatics, National Institute of Animal Science, RDA, Suwon 441-706, Republic of Korea ]
  • Seog-Kyu Choia [ Institute of Conservation Gyeongju Donggyeong Dog, Dongguk, Gyeongju 780-714, Korea ]
  • Chang-Min Park [ Division of Animal Genomics & Bioinformatics, National Institute of Animal Science, RDA, Suwon 441-706, Republic of Korea ]
  • Sun-Ae Park [ Division of Animal Genomics & Bioinformatics, National Institute of Animal Science, RDA, Suwon 441-706, Republic of Korea ]
  • Yong-Min Cho [ Division of Animal Genomics & Bioinformatics, National Institute of Animal Science, RDA, Suwon 441-706, Republic of Korea ]
  • Dajeong Lim [ Division of Animal Genomics & Bioinformatics, National Institute of Animal Science, RDA, Suwon 441-706, Republic of Korea ]
  • Han-Ha Chai [ Division of Animal Genomics & Bioinformatics, National Institute of Animal Science, RDA, Suwon 441-706, Republic of Korea ]
  • Seung-Hwan Lee [ Division of Animal Genomics & Bioinformatics, National Institute of Animal Science, RDA, Suwon 441-706, Republic of Korea ]
  • Ji-Woong Lee [ Devision of Animal Science, Insti. of Ag. Sci. and Tech., Chonnam National University, Chonnam 500-757, Korea. ]
  • Sang-Soo Sun [ Devision of Animal Science, Insti. of Ag. Sci. and Tech., Chonnam National University, Chonnam 500-757, Korea. ]
  • Bong-Hwan Choi [ Division of Animal Genomics & Bioinformatics, National Institute of Animal Science, RDA, Suwon 441-706, Republic of Korea ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

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