Chang Sup Kim, Jeong Hyun Seo, Jae Kyung Sohng, Hyung Joon Cha
언어
영어(ENG)
URL
https://www.earticle.net/Article/A192703
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원문정보
초록
영어
Carbohydrate microarray might have powerful potential for assaying substrate specificity or activity of carbohydrate-processing enzymes such as glycosyltransferase and glycosidase. However, carbohydrate microarray system has been rarely utilized because complex chemical reactions are demanded for microarray preparation. Recently, we have been developed a simple and cost-effective method for fabrication of carbohydrate microarray which uses the reaction of amine-conjugated carbohydrates with glass surface. In the present work, we employed the developed carbohydrate microarray system to rapidly determine substrate specificity or activity of glycosyltransferase. Pasteurella multocidaoriginated sialyltransferase was used as a target glycosyltransferase because it is a wonderful target to prove the potential of carbohydrate microarray. Collectively, the functional carbohydrate microarray would be a powerful analytical tool to screen substrate specificity or other properties of carbohydrate-processing enzymes.
본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.