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Expression of GA733-Fc fusion protein as a vaccine candidate for colorectal cancer in transgenic plants

첫 페이지 보기
  • 발행기관
    한국당과학회 바로가기
  • 간행물
    한국당과학회 학술대회 바로가기
  • 통권
    2011 한국당과학회 하계 학술대회 (2011.06)바로가기
  • 페이지
    pp.56-57
  • 저자
    Zhe Lu, Yingxue Shao, Jung-Hwan Lee, Deuk-Soo Kim, Jun-Sik Yoon, Se-Ra Park, Kyung-Jin Lee, Doo-Byoung Oh, Kyung-A Hwang, Young-Kug Choo, Yeon Soo Han, Kisung Ko
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A192700

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원문정보

초록

영어
Colorectal cancer is the third leading cause of cancer-related death in the Korea. It is eager to find a method for preventing and curing colorectal cancer. The tumor-associated antigen GA733 is a cell surface glycoprotein highly expressed in colorectal carcinoma. In this study, GA733 was fused to Fc fragment of human IgG as a vaccine candidate in plant expression system. The fusion protein GA733-Fc can improve their expression in plant cells and induce cellular and humoral immune responses. GA733-Fc is glycosylated, however the N-glycan structures of plant-derived GA733-Fc differ from their parental counterparts. Plant-derived specific N-glycoforms have the potential to induce immunogenicity and allergenic responses in human therapy. Thus, oligomannose glycan structure was tried to be generated through retaining GA733-Fc and GA733 in ER with KDEL sequence. Agrobacterium-mediated transformation was conducted to generate transgenic plant expressing GA733-Fc and GA733. Recombinant protein expression, accumulation and biological activity of GA733-Fc with KDEL (GA733-FcK), GA733-Fc without KDEL (GA733-Fc), and GA733 with KDEL (GA733K) in transgenic plants were compared by western blot, confocal immunofluorescence analysis, ELISA and mouse immunization, respectively.

저자

  • Zhe Lu [ Department of Biological Science, College of Natural Sciences and Biotechnology Institute, Wonkwang University, Iksan, Korea ]
  • Yingxue Shao [ Department of Biological Science, College of Natural Sciences and Biotechnology Institute, Wonkwang University, Iksan, Korea ]
  • Jung-Hwan Lee [ Department of Biological Science, College of Natural Sciences and Biotechnology Institute, Wonkwang University, Iksan, Korea ]
  • Deuk-Soo Kim [ Department of Biological Science, College of Natural Sciences and Biotechnology Institute, Wonkwang University, Iksan, Korea ]
  • Jun-Sik Yoon [ Department of Biological Science, College of Natural Sciences and Biotechnology Institute, Wonkwang University, Iksan, Korea ]
  • Se-Ra Park [ Department of Biological Science, College of Natural Sciences and Biotechnology Institute, Wonkwang University, Iksan, Korea ]
  • Kyung-Jin Lee [ Department of Biological Science, College of Natural Sciences and Biotechnology Institute, Wonkwang University, Iksan, Korea, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea ]
  • Doo-Byoung Oh [ Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea ]
  • Kyung-A Hwang [ Department of Agrofood Resources, National Academy of Agricultural Science, RDA, Suwon, Korea ]
  • Young-Kug Choo [ Department of Biological Science, College of Natural Sciences and Biotechnology Institute, Wonkwang University, Iksan, Korea ]
  • Yeon Soo Han [ Department of Applied Biology, College of Agriculture and Life Science, Chonnam National University, Gwangju, Korea ]
  • Kisung Ko [ Department of Biological Science, College of Natural Sciences and Biotechnology Institute, Wonkwang University, Iksan, Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국당과학회 [Korean Society for Glycoscience]
  • 설립연도
    2006
  • 분야
    의약학>약학
  • 소개
    본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.

간행물

  • 간행물명
    한국당과학회 학술대회
  • 간기
    연간
  • 수록기간
    2006~2022
  • 십진분류
    KDC 517 DDC 614

이 권호 내 다른 논문 / 한국당과학회 학술대회 2011 한국당과학회 하계 학술대회

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