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Expression and purification of anti-colorectal cancer monoclonal antibody CO17-1A in baculovirus infected insect cells

첫 페이지 보기
  • 발행기관
    한국당과학회 바로가기
  • 간행물
    한국당과학회 학술대회 바로가기
  • 통권
    2011 한국당과학회 하계 학술대회 (2011.06)바로가기
  • 페이지
    pp.54-55
  • 저자
    Jung-Hwan Lee, Deuk-Soo Kim, Yingxue Shao, Jun-Sik Yoon, Se-Ra Park, Yong-Seok Le, Yeon-Soo Han, Kyung-A Hwang, Young-Kug Choo, Kisung Ko
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A192699

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원문정보

초록

영어
In this study, we will attempt to analyze the ER localization, glycosylation pattern and anti-tumor activity of KDEL-fused monoclonal antibody. Advantages of the baculovirus insect cell expression system for production of recombinant proteins include high capacity, flexibility, and glycosylation capability. In this study, this expression system was exploited to produce anti-cancer monoclonal antibody (mAb) CO17-1A, which recognizes the antigen GA733 that is highly expressed on the surface membrane of colorectal carcinoma cells. The heavy chain (HC) and light chain (LC) genes of KDEL-fused mAb CO17-1A were cloned under the control of P10 and PPH promoters in the pFastBacTM dual vector, respectively. Gene expression cassettes carrying the HC and LC genes were transposed into a bacmid in Escherichia coli (DH10Bac). The transposed bacmid was transfected to Sf9 insect cells to generate baculovirus expressing mAb CO17-1A. In future, we will study the expression and localization of KDEL-fused mAb CO17-A in baculovirus infected cells by PCR, western blot, enzyme linked immunosorbent assay (ELISA), confocal immunofluorescence, fluorescence-activated cell sorting analysis (FACs) and antibody dependent cell cytotoxicity (ADCC).

저자

  • Jung-Hwan Lee [ Department of Biological Science, College of Natural Sciences and Biotechnology Institute, Wonkwang University, Iksan, Korea ]
  • Deuk-Soo Kim [ Department of Biological Science, College of Natural Sciences and Biotechnology Institute, Wonkwang University, Iksan, Korea ]
  • Yingxue Shao [ Department of Biological Science, College of Natural Sciences and Biotechnology Institute, Wonkwang University, Iksan, Korea ]
  • Jun-Sik Yoon [ Department of Biological Science, College of Natural Sciences and Biotechnology Institute, Wonkwang University, Iksan, Korea ]
  • Se-Ra Park [ Department of Biological Science, College of Natural Sciences and Biotechnology Institute, Wonkwang University, Iksan, Korea ]
  • Yong-Seok Le [ Department of Parasitology, College of Medicine and Frontier Inje Research for Science and Technology, Inje University, Busan, Korea ]
  • Yeon-Soo Han [ Department of Applied Biology, College of Agriculture and Life Science, Chonnam National University, Gwangju, Korea ]
  • Kyung-A Hwang [ Department of Agrofood Resources, National Academy of Agricultural Science, RDA, Suwon, Korea ]
  • Young-Kug Choo [ Department of Biological Science, College of Natural Sciences and Biotechnology Institute, Wonkwang University, Iksan, Korea ]
  • Kisung Ko [ Department of Biological Science, College of Natural Sciences and Biotechnology Institute, Wonkwang University, Iksan, Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국당과학회 [Korean Society for Glycoscience]
  • 설립연도
    2006
  • 분야
    의약학>약학
  • 소개
    본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.

간행물

  • 간행물명
    한국당과학회 학술대회
  • 간기
    연간
  • 수록기간
    2006~2022
  • 십진분류
    KDC 517 DDC 614

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