Earticle

초록

영어

Natural compounds offer interesting pharmacological perspectives for antiviral drug development. In this study, we obtained low-molecular weight fuco-oligosaccharides (LMFOs), ranging from 1,389 to 3,749 Da from the fucoidan of Korean Undaria pinnatifida sporophyll by crude enzyme preparation of Sphingomonas paucimobilis PF-1 (KCTC 11130BP). The fucoidan and LMFOs had potent antiviral activity against herpes simplex virus types 1 (HSV-1) any detectable level of cytotoxicity on Vero cells. Fucoidan and LMFOs contained strong anti-HSV activity with 50% inhibitory (IC50) at 2.43 and 2.65 μg/ml concentration. Interestingly, they also showed inhibitory activity directly against viral particles as observed in a virucidal assay (88.4%, and 70.5% at 5 μg/ml concentration). Respectively, LMFOs exerted anti-HSV activity when pre-incubated with host cells prior to viral infection, suggesting that a main inhibitory could be mediated via prevention of viral entry. The antiviral activity was significantly affected depending on the degree of sulfation ; antiviral activity was significantly reduced upon removal of sulfate groups. From the FT-IR and NMR analysis, sulfate groups were shown to be located at C-4 of (1→3)-linked fucopyranosyl units, and appeared to be very important for the anti-HSV activity of this polymer.

저자

• Woo Jung Kim [ Department of Biotechnology, The Catholic University of Korea ]
• Hang Soo Jo [ Department of Biotechnology, The Catholic University of Korea ]
• Won Jong Jang [ Dept. of Microbiology, College of Medicine, Kon-Kuk University ]
• Seong Jae Jang [ Department of Biotechnology, The Catholic University of Korea ]
• Andriy Synytsya [ Department of Carbohydrate Chemistry and Technology, Institute of Chemical Technology in Prague, Technicka ]
• Ji Sun Lee [ Department of Biotechnology, The Catholic University of Korea ]
• Yong Il Park [ Department of Biotechnology, The Catholic University of Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국당과학회 학술대회

• 간기

  연간

• 수록기간

  2006~2022

• 십진분류

  KDC 517 DDC 614