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Expression Pattern of Gangliosides on the Differentiation of Human Mesenchymal Stem Cells into Dopaminergic Neuron-like Cells

첫 페이지 보기
  • 발행기관
    한국당과학회 바로가기
  • 간행물
    한국당과학회 학술대회 바로가기
  • 통권
    2011 한국당과학회 하계 학술대회 (2011.06)바로가기
  • 페이지
    pp.36-37
  • 저자
    So-Hyun Lee, Dong-Hoon Kwak, Hyung-Keun You, Beom-Su Kim, Kyung-A Hwang, Byoung-Boo Seo, Hwi-cheul Lee, Jae-Sung Ryu, So-Dam Lee, Ju-Taek Lee, Yoon-Ju Na, Su-Bin Lee, Kyu-Tae Chang, Young-Kug Choo
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A192684

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원문정보

초록

영어
Gangliosides, sialic acid-conjugated glycosphingolipids, are involved in cell proliferation and differentiation. This study investigated the possible role of gangliosides in the dopaminergic neuron-like cells of human mesenchymal stem cells (hMSCs). We have reported the in vitro conditions for inducing hMSCs into dopaminergic neuron-like cells. hMSCs were induced in vitro to become dopaminergic neuron-like cells in 12 days by using a cocktail that includes sonic hedgehog and fibroblast growth factors. First, we investigated the characterization of hMSCs through FACS analysis. The mesenchymal stem cell specific markers, CD44 and CD105, were expressed in hMSCs while the hematopoetic markers, CD45 and CD117 were not. The cells developed a neuronal morphology expressing the neuronal markers Map2 and βⅢtubulin. RT-PCR analysis revealed the expression of dopamine specific genes such as TH, Pitx3, Nurr1 and DA transporter. Immunocytochemistry showed that hMSCs expressed GM3, GM2, GD1a, GD3 and GT1b based on tyrosine hydroxylase (TH) expression. Specifically, a significant decrease in GM3 and GT1b expression was observed during dopaminergic neuron differentiation. These results suggest that ganglioside GT1b may play a role in the dopaminergic neuron differentiation and development process of hMSCs.

저자

  • So-Hyun Lee [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Dong-Hoon Kwak [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Hyung-Keun You [ Bone Regeneration Research Institute, Wonkwang University ]
  • Beom-Su Kim [ Bone Regeneration Research Institute, Wonkwang University ]
  • Kyung-A Hwang [ Department of Agrofood Resources, National Academy of Agricultural Science, RDA, Suwon ]
  • Byoung-Boo Seo [ Department of Animal Resources, college of Life & Environmental Sciences, Daegu University ]
  • Hwi-cheul Lee [ National institute of Animal Science Rural Development Administration, Animal Biotechnology Division, Suwon ]
  • Jae-Sung Ryu [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • So-Dam Lee [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Ju-Taek Lee [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Yoon-Ju Na [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Su-Bin Lee [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Kyu-Tae Chang [ National Primate Research Center (NPRC), Korea Research Institute of Bioscience & Biotechnology, Ochang, Chungbuk ]
  • Young-Kug Choo [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국당과학회 [Korean Society for Glycoscience]
  • 설립연도
    2006
  • 분야
    의약학>약학
  • 소개
    본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.

간행물

  • 간행물명
    한국당과학회 학술대회
  • 간기
    연간
  • 수록기간
    2006~2022
  • 십진분류
    KDC 517 DDC 614

이 권호 내 다른 논문 / 한국당과학회 학술대회 2011 한국당과학회 하계 학술대회

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