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Myogenesis of myoblast C2C12 is regulated through O-GlcNAc modification on transcription factor for myogenin
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- 발행기관
- 한국당과학회 바로가기
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- 간행물
- 한국당과학회 학술대회 바로가기
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- 통권
- 2011 한국당과학회 하계 학술대회 (2011.06)바로가기
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- 페이지
- pp.33-33
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- 저자
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- 언어
- 영어(ENG)
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- URL
- https://www.earticle.net/Article/A192682
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원문정보
초록
- 영어
- β-O-linked N-acetylglucosamine (O-GlcNAc) is dynamic post-translational modification in nucleus and cytosol. O-GlcNAc transferase (OGT) and O-GlcNAcase are involved in O-GlcNAc modification. Many proteins such as transcription factors, cytoskeletal proteins and proteins of variable signal transduction pathway are modified with O-GlcNAc at their Ser/Thr residues and their functions are affected by O-GlcNAc. The hexosamine biosynthesis pathway (HBP) consumes about 5% of glucose in total glucose flux, and it's final product is UDP-GlcNAc, which is source of O-GlcNAc modification. It is reported that O-GlcNAc modification is involved in skeletal muscle metabolism and development process. We studied whether O-GlcNAc modification is involved in differentiation in myoblast C2C12 cell lines. Myoblast C2C12 is differentiated to myotube in differentiation media for 4-5 days. We observed that total O-GlcNAc modification level was dynamically changed during myogenesis. During first 24 hours after induction of myogenesis, total O-GlcNAc modification level decrease and increase again. Also, we treated NAG-thiazoline derivative, specific O-GlcNAcase inhibitors and observed the block of the decrement of total O-GlcNAc modification and inhibition of myotube formation and expression of myogenic markers. Especially, after treatment NAG-thiazoline derivative, we observed that myogenin expression level decreased. So, we checked the myogenin protein stability using MG132 and mRNA level of myogenin using RT PCR. Even after treatment of MG132, the result of the experiment is same as previous experiment, thus myogenin protein stability is not involved with decrease of myogenin expression after treatment NAG-thiazoline derivative. And using RT PCR, we found that mRNA level of myogenin decrease after treatment NAG-thiazoline derivative. So we conclude that O-GlcNAc modification affect the transcription of myogenin.
저자
간행물 정보
발행기관
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- 발행기관명
- 한국당과학회 [Korean Society for Glycoscience]
- 설립연도
- 2006
- 분야
- 의약학>약학
- 소개
- 본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.
간행물
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- 간행물명
- 한국당과학회 학술대회
- 간기
- 연간
- 수록기간
- 2006~2022
- 십진분류
- KDC 517 DDC 614
이 권호 내 다른 논문 / 한국당과학회 학술대회 2011 한국당과학회 하계 학술대회
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