Antimicrobial Activity of Low Molecular Weight Chitosan derived from the Hydrolysis of High Molecular Weight Chitosan by A Chitosanolytic Microorganism Bacillus subtillis JH-1122
Bon-Geun Koo, Gu Baek, Min Jung Kim, Seo Yoon Shin, Yong Il Park, Jae Kweon Park
언어
영어(ENG)
URL
https://www.earticle.net/Article/A192674
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
영어
Chitinolytic and chitosanolytic microorganisms are widely distributed in nature. Biological and enzymatic activities of these enzymes are applied to produce hydrolytic oligosaccharides which have various biological functions involved in anticancer, antimicrobial activities, immune-stimulating activities and so on. In the present study, we describe the biological characteristics including the diversity based on 16S rDNA sequence analysis, production of chitosanase and its hydrolysates, and enzyme activities. Among 15 species of chitosanolytic microorganisms isolated in this study, one named as Bacillus subtilis JH-1122 based on 16s rDNA sequence homology search has shown the highest similarity with most of sub-species of Bacillus. Various sizes of chitosan hydrolysates (Low Molecular Weight Chitosan, LMWC) ranging from 3 to 60 kDa were prepared to assess the effect of molecular weight on antimicrobial activity by enzymatic digestion of chitosan using chitosanase isolated and partially purified from the culture medium of Bacillus subtillis JH-1122. Especially it has shown the higher antimicrobial activity toward antibiotics resistant microorganisms such as Escherichia coli, methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa. These results suggest that LMWC may be a predominant factor either directly or indirectly affecting on broad antimicrobial activities.
저자
Bon-Geun Koo [ Department of Pharmaceutical Science, Gachon University of Medicine and Science ]
Gu Baek [ Department of Pharmaceutical Science, Gachon University of Medicine and Science ]
Min Jung Kim [ Department of Pharmaceutical Science, Gachon University of Medicine and Science ]
Seo Yoon Shin [ Department of Pharmaceutical Science, Gachon University of Medicine and Science ]
Yong Il Park [ Department of Biotechnology and Biomaterial Engineering Research Center, The Catholic University of Korea, Bucheon, Gyeonggi-do 420-743, Korea ]
Jae Kweon Park [ Department of Pharmaceutical Science, Gachon University of Medicine and Science ]
본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.