The anti-complementary substance is able to prevent hemolytic activity by the activation or inhibition of the complement system. Some anti-complementary polysaccharides have been isolated from bacteria, fungi, and plants. In order to develope new mushrooms available as functional food with excellent immuno-modulating activity, we investigated the anti-complementary activities of water soluble polysaccharides extracted from fruiting bodies of 5 mushrooms which have known as traditional medicinal mushrooms for a long time. It was observed that the crude polysaccharides of JFMF mushroom had a potent anti-complementary activity. Crude polysaccharide of JFMF was isolated into a neutral polysaccharide (JFMF-NP) and three acidic polysaccharides (JFMF-AP1, AP2, AP3) by DEAE-sepharose column chromatography. JFMF-AP2, one of these, showed the very excellent anti-complementary activity of ITCH (inhibition of total complement hemolysis) value 70% on 5 ug/ml concentrations. Its molecular weight was estimated less about 5,000 by Toyopearl HW-65F gel filtration chromatography using pullulan molecular size standard kits. Also, detection of a cleavage of C3 by immunoelectrophoresis indicated that JFMF-NP and JFMF-AP2 activates the complement via both the alternative and classical pathways.
저자
Sang-Hong Yoon [ National Academy of Agricultural Science, Suwon 441-707, Korea ]
본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.