Understanding of the molecular relationships in carbohydrate-protein interactions provides useful information on biological processes in living organisms and is also helpful for development of potent biomedical agents. Herein, the interaction unbinding force between GM1 pentasaccharide and Vibrio cholera toxin (ctx) proteins was measured by using atomic force microscopy (AFM), which enabled us to determine the interaction of ctx holotoxin (ctxAB) with GM1 and the interactive From the analogue analyses, we understood how structural and binding positional differences in complex carbohydrate affect the interaction with protein and surmise that the GM1-ctxAB complex makes a “two-finger grip” formation through the conformational change of a flexible carbohydrate. Inconclusion, using AFM force analysis, we successfully quantified and characterized the interactive configuration of carbohydrate-protein molecules.
저자
Jeong Hyun Seo [ Department of Chemical Engineering / Ocean Science and Technology Institute, Pohang University of Science and Technology, Pohang ]
Chang Sup Kim [ Department of Chemical Engineering / Ocean Science and Technology Institute, Pohang University of Science and Technology, Pohang ]
Hyung Joon Cha [ Department of Chemical Engineering / Ocean Science and Technology Institute, Pohang University of Science and Technology, Pohang ]
본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.