Earticle

초록

영어

Natural products have played major roles in drug discovery including development of anti-cancer agents. Especially, saponins have been reported to have important anti-cancer properties. Platycodin D (PD) is a triterpenoid saponin isolated from the root of Platycodon grandiflorum (Campanulaceae). In the present study, the detailed signaling pathway involved in PD-induced apoptosis was investigated in human gastric adenocarcinoma AGS cells. We found that PD treatment resulted in a dose- and time-dependent decrease in the viability of AGS cells. The anti-proliferative effects of PD were accompanied by a G(1) phase cell cycle arrest and apoptosis both in doseand time-dependent manners. The characteristics of apoptosis were further determined by DAPI staining, DNA fragmentation assay, Annexin-V/PI double staining induced by exposure ratio of phosphatidylserine (PS) to the cell surface and sub-G(1) phase ratio. The apoptosis induced by PD was associated with the activation of initiator caspase-8 and -9, as well as the effector caspase-3. PD stimulated Bid cleavage, indicating that the apoptotic action of caspase-8-mediated Bid cleavage leads to the activation of caspase-9. Moreover, PD induced an activation of the phosphorylation of c-Jun N-terminal kinase (JNK), but not p38 and ERK1/2. These results indicate that PD induces cellular apoptosis in AGS cell lines by the activation of caspase cascade and can be a potent anticancer agent against human gastric cancer.

저자

• Jaemoo Chun [ College of Pharmacy, Seoul National University, Seoul 151-742, Korea ]
• Eun-Ji Joo [ College of Pharmacy, Seoul National University, Seoul 151-742, Korea ]
• Minseok Kang [ College of Pharmacy, Seoul National University, Seoul 151-742, Korea ]
• Yeong Shik Kim [ College of Pharmacy, Seoul National University, Seoul 151-742, Korea ] Corresponding author

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간행물

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• 간행물명

  한국당과학회 학술대회

• 간기

  연간

• 수록기간

  2006~2022

• 십진분류

  KDC 517 DDC 614