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Effect of KAI1(CD82) sialylation on the metastatic behavior of human colon cancer cells

첫 페이지 보기
  • 발행기관
    한국당과학회 바로가기
  • 간행물
    한국당과학회 학술대회 바로가기
  • 통권
    2011 한국당과학회 동계 학술대회 (2011.01)바로가기
  • 페이지
    pp.94-95
  • 저자
    Jung-Jin Park, Minyoung Lee, Yeung Bae Jin, Yoon-Jin Lee, Young-Gyu Ko, Yun-Sil Lee
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A192658

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원문정보

초록

영어
The cell membrane glycoprotein KAI1 (CD82) is a candidate metastasis suppressor gene that has been indicated in the progression of solid tumors. KAI1 is significantly downregulated in various human malignancies. However, the expression and mechanism of regulation of KAI1 in metastasis has not yet been fully elucidated. Previously, it is suggested that N-glycosylation of KAI1 occurred at high level and lead to significant inhibition or promotion of cell motility depending on the extracellular matrix. In particular, little is known about the glycosylation of KAI1 and its functional role in cancer metastasis. Recently, we demonstrated that expression of ST6 Gal I, which was highly expressed in colon cancer, was responsible for integrin 1-mediated adhesion and migration in human colon cancer. Remarkably, KAI1 inhibits the tumor metastasis via interaction with tetraspanins, integrins and chemokines which are responsible for cell migration, adhesion and signalling. We found for the first time that KAI1 was sialylated by ST6 Gal I in human colon cancer cells and sialic acid modification was involved in stabilization of KAI1 by proteasome-dependent manner. Also, abolishment of Asn residues largely disrupts the migration-, invasion-, suppressive activities of KAI1. Importantly, when KAI1 was expressed in HCT116 human colon cancer cells with ST6 Gal I, inhibitory effects on migration and invasion was abrogated by altering of p130CAS-Crk coupling, a signaling step for integrin dependent migration. Moreover, sialylated KAI1 interacted with PKC-integrin and is unlikely to inhibit cell migration through its associated proteins. These new observations may indicated that KAI1 can be stably expressed by sialylation in the process of tumor malignancy and sialylation profoundly affects KAI1, leading to significant alterations of functions as metastasis suppressor.

저자

  • Jung-Jin Park [ Division of Radiation Effects, Korea Institute of Radiological and Medical Sciences, School of Life Sciences and Biotechnology, Korea University ]
  • Minyoung Lee [ Division of Radiation Effects, Korea Institute of Radiological and Medical Sciences ]
  • Yeung Bae Jin [ Division of Radiation Effects, Korea Institute of Radiological and Medical Sciences ]
  • Yoon-Jin Lee [ Division of Radiation Effects, Korea Institute of Radiological and Medical Sciences ]
  • Young-Gyu Ko [ School of Life Sciences and Biotechnology, Korea University ]
  • Yun-Sil Lee [ College of Pharmacy & Division of Life & Pharmaceutical Sciences, Ewha Womans University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국당과학회 [Korean Society for Glycoscience]
  • 설립연도
    2006
  • 분야
    의약학>약학
  • 소개
    본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.

간행물

  • 간행물명
    한국당과학회 학술대회
  • 간기
    연간
  • 수록기간
    2006~2022
  • 십진분류
    KDC 517 DDC 614

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