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Structure of fucoidan isolated from sporophyll of Korean Brown Seaweed Undaria pinnatifida

첫 페이지 보기
  • 발행기관
    한국당과학회 바로가기
  • 간행물
    한국당과학회 학술대회 바로가기
  • 통권
    2011 한국당과학회 동계 학술대회 (2011.01)바로가기
  • 페이지
    pp.81-82
  • 저자
    Woo Jung Kim, Andriy Synytsya, Doo Jin Choi, Hang Soo Jo, Ji Sun Lee, Ji Won Choi, Yong-Il Park
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A192648

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원문정보

초록

영어
Polysaccharides from brown seaweeds are interesting due to their various biological activities. A water-soluble polysaccharide was isolated and purified from the sporophyll of Korean Undaria pinnatifida by dilute acid extraction, CaCl2 precipitation to remove alginates, and purified DEAE-cellulose chromatography. Structure of this polysaccharide was characterized by various methods such as elemental analysis, HPAEC-PAD, HPLC analysis, FT-IR, FT-Raman, and 1H and 13C NMR. The average molecular mass of this polysaccharide was estimated about 2.1×103 kDa by HPLC. The polymer showed lower electrophoretic mobility (RSH 0.127) than carrageenans (0.096-0.100) and chondroitin sulphate A (0.103), but higher than alginate (0.170). It is consisted of neutral sugar (52.34 % in mass) and sulfate esters (7.4 % in mass), no uronic acid was observed. From the HPAEC-PAD analysis, the monosaccaride composition was shown to be fucose, galactose, xylose, and mannose, with a molar ratio of 1, 0.8, 0.08, and 0.005, respectively. The major monosaccharide was fucose (50.9 mol%), suggesting that it is a fucoidan, a general name of seaweeds polysaccharides containing fucose residues. Other sugars, galactose (44.6 mol%), xylose (4.2 mol%), and mannose (0.3 mol%), were present as minor component. Very intense and broad FT-IR band at 1258-1259 cm-1 were attributed to S=O stretching of sulfate esters. The IR band centered at 840-842 cm-1 was attributed to COS bending of sulfate substituents. Elemental analysis (EA) of this polysaccharide resulted in C (23.03%), H (3.07%) and S (9.18 %). Taken together, these results clearly demonstrated the presence of sulfate groups in this polysaccharide. The degree of sulfation was determined to me 0.93 by DS (moles of SO3- per mole of sugar units) value, which shows about one sulfate group per sugar unit. Consequently the purified polysaccharide was defined as O-acetylated sulphated galactofucan.

저자

  • Woo Jung Kim [ Department of Biotechnology, The Catholic University of Korea ]
  • Andriy Synytsya [ Department of Carbohydrate Chemistry and Technology, Institute of Chemical Texhnology in Prague ]
  • Doo Jin Choi [ Department of Biotechnology, The Catholic University of Korea ]
  • Hang Soo Jo [ Department of Biotechnology, The Catholic University of Korea ]
  • Ji Sun Lee [ Department of Biotechnology, The Catholic University of Korea ]
  • Ji Won Choi [ Department of Biotechnology, The Catholic University of Korea ]
  • Yong-Il Park [ Department of Biotechnology, The Catholic University of Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국당과학회 [Korean Society for Glycoscience]
  • 설립연도
    2006
  • 분야
    의약학>약학
  • 소개
    본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.

간행물

  • 간행물명
    한국당과학회 학술대회
  • 간기
    연간
  • 수록기간
    2006~2022
  • 십진분류
    KDC 517 DDC 614

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