Chang-Hee Cho, Yong-Sam, Hyang-Sook Yoo, Jeong-Heon Ko
언어
영어(ENG)
URL
https://www.earticle.net/Article/A192644
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원문정보
초록
영어
The development of cancer biomarker is of a great promise to conquer cancer since it leads to an early detection and provide an opportunity for better cancer treatment. Validation is a time-consuming step for biomarker developments requiring investigation of biomarker candidates using thousands or more of biosamples, which is why a sensitive, multiplexing validation method is necessary. To address this challenge, we are developing as antibody-based validation method, in which antibody with oxidized N-glycans was covalently linked to an identifiable DNA tag for use of the template in the transcription by T7 polymerase. The transcripts were designed for analysis on an oligonucleotide chip, where oligonucleotides with complementary sequences were embedded. The transcripts will be quantified and used for an indicator of the amounts of the antigen bound to the DNA-tagged antibody. This strategy will be multiplexed for an establishment of a novel validation method
저자
Chang-Hee Cho [ Deajeon-KRIBB-FHCRC Research Cooperation Center, KRIBB ]
Yong-Sam [ Deajeon-KRIBB-FHCRC Research Cooperation Center, KRIBB ]
Hyang-Sook Yoo [ Deajeon-KRIBB-FHCRC Research Cooperation Center, KRIBB ]
Jeong-Heon Ko [ Deajeon-KRIBB-FHCRC Research Cooperation Center, KRIBB ]
본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.