Mausumi Adhya, Kwang-Sik Choi, Younghun Yu, Moonjae Cho
언어
영어(ENG)
URL
https://www.earticle.net/Article/A192632
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원문정보
초록
영어
A novel sialic acid-specific lectin (MCsialec) was detected from an expressed sequenced tag (EST) sequence from manila clam hemocytes infected with Perkinsus olseni (Kang et al. 2006, Dev. Comp. Immunol. 30, 1119–1131). The cDNA of the lectin was cloned using gene-specific primers based on a previously determined EST and characterized. The full-length cDNA of MCsialec is 603bp in length and encodes a polypeptide of 200 amino acids with a calculated molecular mass of 21.928 kDa. Sequence alignment and protein motif analyses showed that MCsialec shares identity with sialic acid-specific invertebrate lectins from Cepaea hortensis, Helix pomatia, and Haliotis discus discus. The lectin was expressed in E.coli M15cells and purified using a Ni-NTA His-binding resin matrix for antibody production. The presence of the lectin in various tissues of Perkinsus-infected and -uninfected manila clams was analyzed by both PCR and immunohistochemical localization assays. MCsialec was detected in each tissue of the clams; however, upon infection, the level of expression of the lectin increased in each tissue. Vibrio tapetis infection also induced high-level expression of MCsialec in the hemocytes. These data suggest that MCsialec plays a crucial role in the immune system of the manila clam during pathogenic infection.
저자
Mausumi Adhya [ Biochemistry Lab, Department of Medicine, School of Medicine, Department of Physics ]
Kwang-Sik Choi [ School of Applied Marine Science ]
Younghun Yu [ Department of Physics ]
Moonjae Cho [ Biochemistry Lab, Department of Medicine, School of Medicine, Institute of Medical Science, Cheju National University ]
본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.