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Regulatory roles of human leukocyte in the ganglioside expression of the cultured micro-pig aortic endothelial cells

첫 페이지 보기
  • 발행기관
    한국당과학회 바로가기
  • 간행물
    한국당과학회 학술대회 바로가기
  • 통권
    2011 한국당과학회 동계 학술대회 (2011.01)바로가기
  • 페이지
    pp.58-60
  • 저자
    Jin Hyoung Cho, Kyu Tae Chang, Young Choon Lee, Keon Bong Oh, Dong Hoon Kwak, Ji Su Kim, Jae Sung Ryu, So Dam Lee, So Hyun Lee, Ju Taek Lee, Young Kug Choo
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A192629

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원문정보

초록

영어
Gangliosides are ubiquitous component of the membranes of mammalian cells that have been suggested to play important roles in various cell functions such as cell-cell interaction, adhesion, cell differentiation, growth control and signaling. However, the role that gangliosides play in immune rejection response by xenotransplantation is not yet clearly understood. In this study, regulatory roles of human leukocyte in the ganglioside expression of the primary cultured micro-pig aortic endothelial cells (PAECs) were investigated. To study whether human leukocyte are related to expressional changes of gangliosides in PAECs we performed high-performance thin layer chromatography (HPTLC) in PAECs incubated with fetal bovine serum (FBS), as control, or with recombinant human tumor necrosis factor-α (TNF-α), or with human serum and FBS contained with human leukocyte. Both HPTLC and immunohistochemistry analyses revealed that PAECs incubated with FBS are contained GM3, GM1 and GD3 as major gangliosides. However, ganglioside GM1 significantly decreased in PAECs incubated for 5hrs with TNF-α (10 ng/ml), or with 10% human serum and FBS contained with human leukocytes. Taken together, these results suggest that human TNF-α secreted by human leukocyte against PAECs and possibly happened expressional pattern changes of ganglioside GM1 in PAECs by secreted TNF-α. The finding that human leukocyte promotes expressional pattern changes of ganglioside in PAECs depending on TNF-α secreation may be relevant for designing future therapeutic strategies intended to prolong xenograft survival.

저자

  • Jin Hyoung Cho [ Division of Biological Science, College of National Sciences, Wonkwang University ]
  • Kyu Tae Chang [ National Primate Research Center, Korea Research Institute of Bioscience & Biotechnology, Ochang ]
  • Young Choon Lee [ Department of Biotechnology and Biological Science, Dong-A University, Pusan ]
  • Keon Bong Oh [ National Institute of Animal Science Rural Development Administration, Animal Biotechnology Division, Suwon ]
  • Dong Hoon Kwak [ Division of Biological Science, College of National Sciences, Wonkwang University ]
  • Ji Su Kim [ National Primate Research Center, Korea Research Institute of Bioscience & Biotechnology, Ochang ]
  • Jae Sung Ryu [ Division of Biological Science, College of National Sciences, Wonkwang University ]
  • So Dam Lee [ Division of Biological Science, College of National Sciences, Wonkwang University ]
  • So Hyun Lee [ Division of Biological Science, College of National Sciences, Wonkwang University ]
  • Ju Taek Lee [ Division of Biological Science, College of National Sciences, Wonkwang University ]
  • Young Kug Choo [ Division of Biological Science, College of National Sciences, Wonkwang University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국당과학회 [Korean Society for Glycoscience]
  • 설립연도
    2006
  • 분야
    의약학>약학
  • 소개
    본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.

간행물

  • 간행물명
    한국당과학회 학술대회
  • 간기
    연간
  • 수록기간
    2006~2022
  • 십진분류
    KDC 517 DDC 614

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