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Ganglioside GD1a expression in colon SW620 cancer cells were treated with the monoclonal antibody against EpCAM

첫 페이지 보기
  • 발행기관
    한국당과학회 바로가기
  • 간행물
    한국당과학회 학술대회 바로가기
  • 통권
    2011 한국당과학회 동계 학술대회 (2011.01)바로가기
  • 페이지
    pp.38-39
  • 저자
    Dong Hoon Kwak, Jae-Sung Ryu, So-dam Lee, Ju Taek Lee, Jin Hyung Cho, Kisung Ko, Kyung-A Hwang, Young-Kug Choo
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A192628

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원문정보

초록

영어
The epithelial cell adhesion molecule (EpCAM) was initially described the human colorectal carcinoma-associated GA733 antigen as a cell surface protein selectively expressed in some myeloid cancers. Gangliosides are sialic acid-containing glycosphingolipids involved in inflammation and oncogenesis. We have demonstrated that treatment with anti-EpCAM mAb and RAW264.7 cells significant inhibited the cell growth in SW620 cancer cells, but neither anti-EpCAM mAb nor RAW264.7 cells alone induced cytotoxicity. The relationship between ganglioside expression and the anti-cancer effects of anti-EpCAM mAb and RAW264.7 was investigated by high-performance thin-layer chromatography. The results demonstrated that GD1a play important roles in the ability of anti-EpCAM to inhibit cell growth in SW620 cells. Anti-EpCAM mAb treatment increased the expression of anti-apoptotic proteins such as Bcl-2, but the expression of pro-apoptotic proteins were unaltered. We observed that anti-EpCAM mAb significantly inhibited the growth of colon tumors, as determined by a decrease in tumor volume and weight. We observed that anti-EpCAM mAb significantly inhibited the growth of colon tumors, as determined by a decrease in tumor volume and weight. Immunohistochemical analysis revealed that anti-EpCAM mAb at a dose of 100 mg/mouse inhibited tumor cell growth. The expression of pro-apoptotic proteins and GD1a were increased by treatment with anti-EpCAM mAb. In light of these results, further clinical investigation should be conducted on anti-EpCAM mAb to determine its possible chemopreventive and/or therapeutic efficacy against human colon cancer.

저자

  • Dong Hoon Kwak [ Department of Biological Science and Institute of Biotechnology Wonkwang University ]
  • Jae-Sung Ryu [ Department of Biological Science and Institute of Biotechnology Wonkwang University ]
  • So-dam Lee [ Department of Biological Science and Institute of Biotechnology Wonkwang University ]
  • Ju Taek Lee [ Department of Biological Science and Institute of Biotechnology Wonkwang University ]
  • Jin Hyung Cho [ Department of Biological Science and Institute of Biotechnology Wonkwang University ]
  • Kisung Ko [ Department of Biological Science and Institute of Biotechnology Wonkwang University ]
  • Kyung-A Hwang [ Department of Agrofood Resources, National Academy of Agricultural Science, RDA ]
  • Young-Kug Choo [ Department of Biological Science and Institute of Biotechnology Wonkwang University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국당과학회 [Korean Society for Glycoscience]
  • 설립연도
    2006
  • 분야
    의약학>약학
  • 소개
    본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.

간행물

  • 간행물명
    한국당과학회 학술대회
  • 간기
    연간
  • 수록기간
    2006~2022
  • 십진분류
    KDC 517 DDC 614

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