Earticle

초록

영어

In order to obtain feasibility data regarding the possibility of using chondroitin sulfate (CS) in drug delivery system, CS was chemically modified by a one-step process with acetylation. Although 3 polymers with different degrees of acetylation were synthesized, only the sample with the highest degree of acetylation (AC-CS3) was tested as a nanogel because the others (AC-CS1 and 2) dissolved in distilled water (DW) in the test range. The AC-CS3 nanocarrier was characterized by fluorescence probe and dynamic light scattering (DLS) techniques. Its critical aggregation concentration (CAC) was < 2.0 × 10−2 mg/ml at 25 °C. The partition equilibrium constant, Kv, of the nanoparticle (7.88 × 105) was similar to that of polymeric micelles, which means that the acetyl group may act as a hydrophobic core controlling pharmacokinetic behavior. The higher surface charge value in the nanoparticle, above -40 due to carboxyl and sulfate groups in CS, explains its good stability. The anticancer drug doxorubicin (DOX) loading efficiency of the AC-CS3 nanoparticle was also superior, at above 90%. Changes in the size of the polydispersion index (PDI) of nanoparticle loaded with DOX over a 3-week period were negligible. The nanoparticle interacted with HeLa cells and were internalized together with the entrapped drug within the cytoplasm, probably via an endocytic mechanism exploited by sugar receptors. Based on these results, the AC-CS3 nanogel is expected to prove useful as an anti-cancer drug carrier for chemotherapy.

저자

• Wooram Park [ Department of Biotechnology, The Catholic University of Korea ]
• Kun Na [ Department of Biotechnology, The Catholic University of Korea ]

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간행물

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• 간행물명

  한국당과학회 학술대회

• 간기

  연간

• 수록기간

  2006~2022

• 십진분류

  KDC 517 DDC 614