Dynamic protein modification, called post-translational modification (PTM), is the chemical modification of a protein and an important biological regulatory mechanism to understand cellular function and interactions. Glycosylation is one of the most common post-translational modifications of proteins and is involved in many physiological functions and biological pathways. Analysis of glycoproteins including site mapping and glycan characterization is an essential sub-field of proteomics using high throughput techniques by mass spectrometry. Recent improvements in mass spectrometry provide sensitive methodologies by liquid chromatography tandem mass spectrometry (LC-MS/MS) as an innovative analytical technology applicable to a wide number of analyses in glycoproteins. In addition to mapping sites of N-linked glycosylation, mapping sites of O-linked glycosylation are another current challenge in proteomics. Here we demonstrate that the methodologies allow for analyses of glycoproteins and their mapping sites in various biological species and also discuss the effective uses of mass spectrometry for glycomic analysis and modern techniques of biomolecular mass spectrometry with the glycome from glycoproteins.
저자
Jae-Min Lim [ Department of Chemistry, Changwon National University ]
Yong-Ill Lee [ Department of Chemistry, Changwon National University ]
Lance Wells [ Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens, GA 30602, U.S.A. ]
본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.