Earticle

초록

영어

A novel polysialic acid-specific endo-sialidase referred to Endo-PS was purified from the culture filtrate of Pseudomonas fluorescens JK-0412 to apparent homogeneity and has a subunit molecular mass of 20 kDa. The purified enzyme Endo-PS has a molecular weight of 110 kDa by native-PAGE, suggesting that the active enzyme is a hexamer. Although 12 residues of N-terminal amino acid sequence of Endo-PS showed 75% identity with 21-kDa chitin binding protein CBP21 of Serratia marcescens 2170, no significant homology with other known proteins was observed. The specific activity of Endo-PS toward an artificial substrate 4-methylumbelliferyl-sialic acid (4-MU-Neu5A) was determined to be 16 nmole/mg/min with Km value of 0.08 mM. The enzyme was maximally active at an optimal temperature of 37℃ and pH 8. It possesses the ability to only hydrolyze natural substrate α2-8 linked homo-polysialic acid (or colominic acid), but not α2-3 or α2-6 sialyllactoses. Under optimal condition, sialyloligosaccharides ranging from dimer up to 30 residues long liberated from the cleavage of polysialic acid in the function of time were identified by high performance anion-exchange chromatography (HPAEC). Taken together these results suggest that the biochemical properties of Endo-PS purified from the culture filtrate of P. fluorescens JK-0412 are specificfor polysialic acid, referred as a novel endo-polysialidase, reporting first about the properties of non-pathogenic bacterial-derived endo-sialidase.

키워드

저자

• Doo Jin Choi [ Dept. of Biotechnology, The Catholic University of Korea ]
• Jae Kweon Park [ Dept. of Biotechnology, The Catholic University of Korea ]
• Sung Min Kim [ Dept. of Biotechnology, The Catholic University of Korea ]
• Woo Jung Kim [ Dept. of Biotechnology, The Catholic University of Korea ]
• Hye Ran Moon [ Dept. of Biotechnology, The Catholic University of Korea ]
• Yong Il Park [ Dept. of Biotechnology, The Catholic University of Korea ]

참고문헌

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간행물

표지보기 관심저널 등록

• 간행물명

  한국당과학회 학술대회

• 간기

  연간

• 수록기간

  2006~2022

• 십진분류

  KDC 517 DDC 614