Earticle

초록

영어

Fucoidans, a group of marine sulfated polysaccharides of the cell-wall matrix of brown algae, exhibit a wide range of biological activities, such as anti-inflammatory, antiviral, antioxidant, anticoagulant, antitumor, and anti-angiogenesis activities. In spite of their interest as biologically active compounds, no convenient commercial sources with fucoidanolytic activities are available yet. we have searched for a bacterial strain capable of degrading a fucoidan which was purified in our lab from the sporopyll of Korean Undaria pinnatifida. Among isolates, a strain, tentatively named as Sphingomonas paucimobilis PF-1 (KCTC 11130BP) fucoidanase activity producing low-molecular weight fuco-oligosaccharides (LMFOs) ranging from 1,389 to 3,749 Da was partially purified from Sphingomonas paucimobilis PF-1. Resulting LMFOs were investigated for immunomodulating activity on murine macrophages and splenocytes. Treatment of fucoidan and LMFOs resulted in inhibition of the growth of murine macrophage RAW 264.7 cells, but its cytotoxicity was not observed in normal murine splenocytes. Treatment of fucoidan and LMFOs induced TNF-a in a dose-dependent manner from two types of macrophages, RAW 264.7 cells and murine peritoneal macrophages. The TNF-a-inducing activity of LMFOs was higher than that of fucoidan. LMFOs also activated murine splenocytes to produce cytokine (IL-6) and chemokines (RANTES and MIP-1a). These results indicate that fucoidan and LMFOs stimulate early innate immune system with no cytotoxicity.

저자

• Woo Jung Kim [ Dept. of Biotechnology, The Catholic University of Korea ]
• Yung Choon Yoo [ Dept. of Microbiology, Konyang University, Konyang University Hospital ]
• Yong Il Park [ Dept. of Biotechnology, The Catholic University of Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국당과학회 학술대회

• 간기

  연간

• 수록기간

  2006~2022

• 십진분류

  KDC 517 DDC 614