Earticle

초록

영어

Lipid rafts are membrane microdomains enriched in saturated phospholipid, sphingolipid, and cholesterol. Pattern Recognition Receptors (PRRs) such as Toll-like Receptors (TLRs), dectin-1 or DC-SIGN are expressed on lipid rafts. SIGN-R1, a PRR, is a homologue of human DC-SIGN and expressed at high levels on macrophages within the marginal zone of the spleen and lymph node medulla, mediating the uptake of pneumococcal capsular polysaccharides (CPSs). Here we showed that SIGN-R1 was expressed in lipid rafts of SIGN-R1 transfectant cell lines in vitro as well as in lipid rafts of spleen or lymphnodes in vivo. It was identified that SIGN-R1 recruited its ligands such as dextran and pneumococcal capsular polysaccharides in vitro. Additionally, it was found that SIGN-R1 was multimerized in lipid rafts with the intravenous administration of S. pneumoniae type 14 or its capsular polysaccharide (CPS14) in vivo. However, the multimerization of SIGN-R1 was not occurred against CPS-depleted S. pneumoniae type 14 or another gram-positive bacteria, Streptococcus aureus. All of these results suggested that the localization of SIGN-R1 in lipid rafts might be important for protecting host against S. pneumoniae, activation its defense mechanism including the multimerization of SIGN-R1. Currently, further studies are in investigation as follows. 1) Whether SIGN-R1 could mediate complement activation against its ligands in lipid rafts? 2) Whether SIGN-R1 mediates a specific signaling from lipid rafts against its ligand? 3) Whether SIGN-R1 mediates the generation of a specific cytokine from lipid rafts against its ligands?

저자

• Jin-Yeon Park [ Department of Biomedical Science & Technology, Institute of Biomedical Science & Technology (IBST), Konkuk University ]
• Woo-Sung Choi [ Department of Biomedical Science & Technology, Institute of Biomedical Science & Technology (IBST), Konkuk University ]
• Heong-jwa Choi [ Department of Biomedical Science & Technology, Institute of Biomedical Science & Technology (IBST), Konkuk University ]
• Miglena G. Prabagar [ Department of Biomedical Science & Technology, Institute of Biomedical Science & Technology (IBST), Konkuk University ]
• Kyeong-Hyeon Kang [ Department of Biomedical Science & Technology, Institute of Biomedical Science & Technology (IBST), Konkuk University ]
• Young-Sun Kang. [ Department of Biomedical Science & Technology, Institute of Biomedical Science & Technology (IBST), Konkuk University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국당과학회 학술대회

• 간기

  연간

• 수록기간

  2006~2022

• 십진분류

  KDC 517 DDC 614