Kyoung-Ro Kim, Dong-Gyun Kang, Young-Hwan Kim, Hyung-Joon Cha
언어
영어(ENG)
URL
https://www.earticle.net/Article/A192599
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원문정보
초록
영어
Cancer that is one of serious diseases for human causes angiogenesis, metastasis, and abnormal cell divisions including genetic change. Thus, lots of researchers have been investigated to inhibit cancer. Human MAC-2 binding protein (or human 90K, h90K) has very crucial role for inhibition of cancer metastasis. h90K is a glycoprotein having 7 N-glycosylation sites. In this work, we performed expression of recombinant h90K glycoprotein in Drosophila S2 cell system. We constructed stably-transfected S2-h90K cells by antibiotic selection and confirmed h90K expression using Western Blot analyses with hexa histidine tag and bioactivity check. And we compared with Chinese Hamster Ovary (CHO) cell-deived human MAC-2 binding protein for expression and activity check. We also identified glycosylation patterns of recombinant h90K glycoprotein using HPLC and MALDI-MS. This results and application technology will contribute for scientific and medical engineering.
저자
Kyoung-Ro Kim [ National Research Laboratory of Molecular Biotechnology and Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH) ]
Dong-Gyun Kang [ National Research Laboratory of Molecular Biotechnology and Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH) ]
Young-Hwan Kim [ Mass Spectrometry Research Team, Korea Basic Science Institute (KBSI) ]
Hyung-Joon Cha [ National Research Laboratory of Molecular Biotechnology and Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH) ]
본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.