Cell-surface glycans are involved in both physiological and pathological processes through their interactions with proteins. Carbohydrate microarrays have become powerful tools for studies of these biomolecular interactions in a high-throughput manner. A major driving force for theexpanded use of glycan microarrays is the development of new experimental protocols in which they can be applied. A potentially important application of these microarrays is to assay glycosyltransferase activities. To date, only a few examples of enzymatic reactions taking place on the carbohydrate microarrays have been reported. Herein, we present the results of our recent studies which show that carbohydrate microarrays can be used for assaying galactosyltransferase activity. The approach requires only atiny amount of immobilized acceptor substrates (picomoles), and enzymatic catalytic activities are readily assessed by measuring the amount of a product formed in a time-dependent manner. The level of time-dependent product conversion is determined by using fluorescence detection of lectin recognition of carbohydrate products.
저자
Sungjin Park [ Department of Chemistry, Yonsei University, Seoul 120-749, Korea ]
Jaeyoung Pai [ Department of Chemistry, Yonsei University, Seoul 120-749, Korea ]
Injae Shin [ Department of Chemistry, Yonsei University, Seoul 120-749, Korea ]
본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.