Baculovirus expression system is considered to have several benefits for production of recombinant protein such as high capacity, flexibility, and similar human glycosylation pattern. Typically, before any large scale expression work was commenced, an accurate titering of recombinant baculovirus is required to ensure the most appropriate expression conditions. One of them, the method have been developed by BaculoELISA Titer Kit (Clontech, Mountain Biew, CA, USA). Stocks of any Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) type baculovirus can be titered using this kit. However, control virus of this kit is high cost and relatively small quantity in kit. In this study, production of mAb CO17-1A by baculovirus infection was performed at different times, concentrations and passages. Immunoblot analysis confirmed expression of mAb CO17-1A in the infected cells. mAb CO17-1A expressed significantly high level under the condition of baculovirus passages 3, 72h and 400㎕ in 5ml insect cells after infection. The results indicated that it is not necessary to use BaculoELISA assay.
저자
Da-young Park [ Department of Biological Science, Wonkwang University, Iksan, Jeonbuk 570-749, Korea ]
Yangkang So [ Department of Biological Science, Wonkwang University, Iksan, Jeonbuk 570-749, Korea ]
Kisung Ko [ Department of Biological Science, Wonkwang University, Iksan, Jeonbuk 570-749, Korea ]
본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.