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Comparison of gangliosides expression patterns between human adipose and dental pulp-derived mesenchymal stem cells differentiation into osteoblast

첫 페이지 보기
  • 발행기관
    한국당과학회 바로가기
  • 간행물
    한국당과학회 학술대회 바로가기
  • 통권
    2010년 한국당과학회 하계학술대회 (2010.06)바로가기
  • 페이지
    pp.63-64
  • 저자
    So-Hyun Lee, Jae-Sung Ryu, Jin-Hyoung Cho, So-Dam Lee, Ju-Taek Lee, Da-Hyun Park, Seul-Yi Lee, Dong Hoon Kwak, Young-Kug Choo
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A192576

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원문정보

초록

영어
Human adipose-derived stem cells (hADSCs) and dental pulp-derived stem cells (hDPSCs) have been considered alternative sources of adult stem cells because of their potential to trans-differentiate into multiple cell lineages. This study investigated the possible role of gangliosides in the osteoblast differentiation of hADSCs and hDPSCs. First, we investigated characterization of hADSCs and hDPSCs using FACS analysis. Mesenchymal stem cell specific markers, CD44 and CD105, were expressed but not hematopoetic markers, CD45 and CD117 in both of hADSCs and hDPSCs. High-performance thin-layer chromatography analysis showed that increased gangliosides were associated with differentiation of hADSCs and hDPSCs into osteoblasts. RT-PCR analysis confirmed that osteoblast specific genes, ALP, BMP-2, collagen were expressed in differentiated osteoblasts, however, the another osteoblast specific gene, osteocalcin, was not expressed. When hADSCs and hDPSCs were cultured under osteoblast- differentiation conditions, alkaline phosphatase (ALP) activity was increased in comparison to hADSCs and hDPSCs. Furthermore, specifically both ALP activity and ganglioside expression increased more in hDPSCs-derived osteoblasts than hADSCs-derived osteoblasts. These results suggest that gangliosides play a more important role in regulating the osteoblast-differentiation of hDPSCs compared to hADSCs.

저자

  • So-Hyun Lee [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan, Jeonbuk ]
  • Jae-Sung Ryu [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan, Jeonbuk ]
  • Jin-Hyoung Cho [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan, Jeonbuk ]
  • So-Dam Lee [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan, Jeonbuk ]
  • Ju-Taek Lee [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan, Jeonbuk ]
  • Da-Hyun Park [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan, Jeonbuk ]
  • Seul-Yi Lee [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan, Jeonbuk ]
  • Dong Hoon Kwak [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan, Jeonbuk ]
  • Young-Kug Choo [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan, Jeonbuk ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국당과학회 [Korean Society for Glycoscience]
  • 설립연도
    2006
  • 분야
    의약학>약학
  • 소개
    본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.

간행물

  • 간행물명
    한국당과학회 학술대회
  • 간기
    연간
  • 수록기간
    2006~2022
  • 십진분류
    KDC 517 DDC 614

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