Earticle

초록

영어

The (1,6)-fucosylation of core N-glycan (core fucose) has been known to play crucial roles in various pathophysiological events such as oncogenesis and metastasis. Therefore, a fluorescent probe recognizing core fucose will be a powerful tool for elucidating its functional role and further diagnosing cancer. The fleA gene, which has been known to code Aspergillus oryzae lectin (AOL), was cloned from A. oryzae RIB40. We fused it with the either citrine or cerulean gene (improved variants of yellow or cyan fluorescent protein) to generate two expression vectors. The resulting fusion gene products (FleA-citrin & FleA–cerulean) were successfully expressed in Escherichia coli and purified homogeneously using two step affinity purification method. The fluorescent FleA fusion proteins were shown to detect core fuose in glycoproteins on the surface of cancer cells. The reduction of core fucose amount induced by the transfection of (1,6)-fucosyltransferase gene, Fut8, siRNA was also successfully detected by the FleA fusion proteins. These results show that our FleA-fluorescent fusion proteins would be employedas a valuable fluorescent probe for detecting core fucose.

저자

• Jiyoung Mun [ Omics and Integration Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Functional Genomics Program, School of Science, University of Science and Technology, Yuseong, Daejeon ]
• Yu Jin Kim [ Omics and Integration Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB) ]
• Kyung Jin Lee [ Omics and Integration Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB) ]
• Eun Mi Jung [ Omics and Integration Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB) ]
• Sun-Wha Kim [ Omics and Integration Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB) ]
• Seung-Goo Lee [ Omics and Integration Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB) ]
• Ohsuk Kwon [ Omics and Integration Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Functional Genomics Program, School of Science, University of Science and Technology, Yuseong, Daejeon ]
• Doo-Byoung Oh [ Omics and Integration Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Functional Genomics Program, School of Science, University of Science and Technology, Yuseong, Daejeon ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국당과학회 학술대회

• 간기

  연간

• 수록기간

  2006~2022

• 십진분류

  KDC 517 DDC 614