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N-Acetylglucosaminyltransferase I (GnTI) is Developmentally essential in Rice (Oryza sativa)

첫 페이지 보기
  • 발행기관
    한국당과학회 바로가기
  • 간행물
    한국당과학회 학술대회 바로가기
  • 통권
    4th 2009 Annual Meeting of Korean Society for Glycoscience in 2009 (2009.11)바로가기
  • 페이지
    pp.54-54
  • 저자
    Wahyu Indra Fanata, Jeong Chan Moon, Joo Mi Jeon, Bo Hwa Son, In Jung Jung, Jae Yong Yoo, Jae Ho Cha, Je Hein Kim, Rikno Harmoko, Ki Seong Ko, Sang Yeol Lee, Kyun Oh Lee
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A192543

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원문정보

초록

영어
N-acetylglucosaminyltransferase I (GnTI) is an enzyme that controls the formation of hybrid and complex type N-glycans. Plant produce complex N-glycans with β1,2-xylose and core α1,3-fucose residues linked to the conserved core oligosaccharide. Production of heterologous glycoprotein in plant for therapeutic purposes is limited by the presence of plant specific oligosaccharide residues that are considered to arouse immunogenic response in the recipient body. Therefore, efforts for developing humanized N-glycosylation system in plants are required for the large scale production of safe therapeutic glycoproteins. In this regard, we isolated a rice mutant lacking GnTI activity. Biochemical analyses showed that rice gnt1 mutant predominantly produced high mannose type glycans and β1,2-xylose and core α1,3-fucose were absent on the endogenous glycoproteins. However, the rice gnt1 mutant showed severe developmental defects such as retarded shoot and root development, failure in tiller formation, and finally resulted in early developmental lethality. Interestingly, callus induced from gnt1 seeds was maintainable and proliferation rate of gnt1 callus was similar with that of WT. However, gnt1 calli were relatively smaller than that of WT. [Supported by EB-NCRC & BK21 program]

저자

  • Wahyu Indra Fanata [ Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University ]
  • Jeong Chan Moon [ Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University ]
  • Joo Mi Jeon [ Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University ]
  • Bo Hwa Son [ Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University ]
  • In Jung Jung [ Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University ]
  • Jae Yong Yoo [ Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University ]
  • Jae Ho Cha [ Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University ]
  • Je Hein Kim [ Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University ]
  • Rikno Harmoko [ Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University ]
  • Ki Seong Ko [ Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University ]
  • Sang Yeol Lee [ Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University ]
  • Kyun Oh Lee [ Division of Applied Life Science (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국당과학회 [Korean Society for Glycoscience]
  • 설립연도
    2006
  • 분야
    의약학>약학
  • 소개
    본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.

간행물

  • 간행물명
    한국당과학회 학술대회
  • 간기
    연간
  • 수록기간
    2006~2022
  • 십진분류
    KDC 517 DDC 614

이 권호 내 다른 논문 / 한국당과학회 학술대회 4th 2009 Annual Meeting of Korean Society for Glycoscience in 2009

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