Earticle

현재 위치 Home

Poster-17

Expression, glycosylation, and function of anti-colorectal cancer monoclonal antibody CO17-1A in insect cells

첫 페이지 보기
  • 발행기관
    한국당과학회 바로가기
  • 간행물
    한국당과학회 학술대회 바로가기
  • 통권
    4th 2009 Annual Meeting of Korean Society for Glycoscience in 2009 (2009.11)바로가기
  • 페이지
    pp.50-50
  • 저자
    Eun-Yi Oh, Mira Song, Mi-Hyun Ahn, Da-Young Park, Kyung-Jin Lee, Yangkang So, Zhe Lu, Kwang-Wok Min, Seul-Ki Lee, Jung-Hwan Lee, Doo-Byoung Oh, Youngkwan Kim, Kisung Ko
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A192532

※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.

원문정보

초록

영어
Insect cell expression system using baculovirus has several benefits from high capacity, flexibility, and safety to humans and glycosylation capability. Thus, the baculovirus insect cell system has been widely used for production of recombinant protein. The HC and LC genes of the mAb CO17-1A were cloned under the control of two different promoters, P10 and PPH, respectively, on baculovirus expression pFastBacTM Dual vector. The gene expression cassettes carrying HC and LC genes were transferred into a parent bacmid in DH10Bac. Immunoflouorescence analyses and Western blot confirmed expression and secretion of mAb CO17-1A in the virus infected insect cells. The optimum condition for mAb expression was optimized at 24, 48 and 72hr after the virus infection with MOI ranging (0.2, 1 and 5). HPLC analysis revealed that the insect-derived mAb CO17-1A had insect specific glycan structures. Cell ELISA showed that the purified mAb from insect cell cultured media had a specific binding activity to SW948 cell. These results indicated that the baculovirus insect cell system is able to express, assemble, and secrete functional full size monoclonal antibody with insect specific glycosylation.

저자

  • Eun-Yi Oh [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Mira Song [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Mi-Hyun Ahn [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Da-Young Park [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Kyung-Jin Lee [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Korean Research Institute of Bioscience & Biotechnology, Daejeon ]
  • Yangkang So [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Zhe Lu [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Kwang-Wok Min [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Seul-Ki Lee [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Jung-Hwan Lee [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Doo-Byoung Oh [ Korean Research Institute of Bioscience & Biotechnology, Daejeon ]
  • Youngkwan Kim [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Kisung Ko [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국당과학회 [Korean Society for Glycoscience]
  • 설립연도
    2006
  • 분야
    의약학>약학
  • 소개
    본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.

간행물

  • 간행물명
    한국당과학회 학술대회
  • 간기
    연간
  • 수록기간
    2006~2022
  • 십진분류
    KDC 517 DDC 614

이 권호 내 다른 논문 / 한국당과학회 학술대회 4th 2009 Annual Meeting of Korean Society for Glycoscience in 2009

    피인용수 : 0건 (자료제공 : 네이버학술정보)

    함께 이용한 논문 이 논문을 다운로드한 분들이 이용한 다른 논문입니다.

      출처 : 네이버학술정보

        0개의 논문이 장바구니에 담겼습니다.

        페이지 저장
        소속기관 조회
        이용자님의 소속기관(단체)이 서비스에 가입되어 있는지 확인해 보십시오.
        기관회원에 소속되어 있는 이용자는 원문을 무료로 이용할 수 있습니다.