Earticle

현재 위치 Home

Poster-16

Cloning and functional characterization of pig CMP-N-acetylneuraminic acid hydroxylase(pCMAH) for the synthesis of N-glycolylneuraminic acid as the xenoantigenic determinant in pig-to-human xenotransplantation

첫 페이지 보기
  • 발행기관
    한국당과학회 바로가기
  • 간행물
    한국당과학회 학술대회 바로가기
  • 통권
    4th 2009 Annual Meeting of Korean Society for Glycoscience in 2009 (2009.11)바로가기
  • 페이지
    pp.49-49
  • 저자
    Kwon-Ho Song, Hyun-Kwon Ko, Seok-Jo Kim, Kyung-Min Kwon, Cheorl-Ho Kim
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A192530

※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.

원문정보

초록

영어
In this study, pig CMP-N-acetylneuraminic acid hydroxylase gene (pcmah), a key enzyme for the synthesis of N-glycolylneuraminic acid was cloned from pig small intestine and characterized. The open reading frame of pcmah was 1,734 bp encoded 577 amino acids and consists of fourteen exons. Organ expression pattern analysis reveals that pig CMAH mRNA is mainly expressed in pig rectum, tongue, spleen, and colon tissues, being the most highly expressed in small intestine. In the ectopic expression of pcmah, when PK15 cells and ECV304 cells were transfected with the cloned pcmah, NeuGc contents of these transfectants were greater in comparison to vector transfectants used as control. In addition, in the functional analysis of NeuGc, human serum-mediated cytotoxicity was elevated in the ectopic NeuGc expressing pcmah-transfected cells compared with controls. Moreover, binding of human IgM to the pcmah-transfected cells was significantly increased, whereas binding of IgG were slightly increased indicating that the human IgM type was a major anti-NeuGc antibody. From the results, it was concluded that the pig CMAH gene was capable to synthesize the NeuGc acting as a xenoantigen in human, confirming the NeuGc-mediated rejection response in pig-to-human xenotransplantati

저자

  • Kwon-Ho Song [ Molecular and Cellular Glycobiology Unit, Department of Biological Science, SungKyunKwan University ]
  • Hyun-Kwon Ko [ Molecular and Cellular Glycobiology Unit, Department of Biological Science, SungKyunKwan University ]
  • Seok-Jo Kim [ Molecular and Cellular Glycobiology Unit, Department of Biological Science, SungKyunKwan University ]
  • Kyung-Min Kwon [ Molecular and Cellular Glycobiology Unit, Department of Biological Science, SungKyunKwan University ]
  • Cheorl-Ho Kim [ Molecular and Cellular Glycobiology Unit, Department of Biological Science, SungKyunKwan University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국당과학회 [Korean Society for Glycoscience]
  • 설립연도
    2006
  • 분야
    의약학>약학
  • 소개
    본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.

간행물

  • 간행물명
    한국당과학회 학술대회
  • 간기
    연간
  • 수록기간
    2006~2022
  • 십진분류
    KDC 517 DDC 614

이 권호 내 다른 논문 / 한국당과학회 학술대회 4th 2009 Annual Meeting of Korean Society for Glycoscience in 2009

    피인용수 : 0건 (자료제공 : 네이버학술정보)

    함께 이용한 논문 이 논문을 다운로드한 분들이 이용한 다른 논문입니다.

      출처 : 네이버학술정보

        0개의 논문이 장바구니에 담겼습니다.

        페이지 저장
        소속기관 조회
        이용자님의 소속기관(단체)이 서비스에 가입되어 있는지 확인해 보십시오.
        기관회원에 소속되어 있는 이용자는 원문을 무료로 이용할 수 있습니다.