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Glycomodification and charaterization of anti-colorectal cancer immunotherapeutic monoclonal antibody in plant

첫 페이지 보기
  • 발행기관
    한국당과학회 바로가기
  • 간행물
    한국당과학회 학술대회 바로가기
  • 통권
    4th 2009 Annual Meeting of Korean Society for Glycoscience in 2009 (2009.11)바로가기
  • 페이지
    pp.47-47
  • 저자
    Yangkang So, Seul-Ki Lee, Da-Young Park, Zhe Lu, Eun-Yi Oh, Kwang-Wook Min, Jung-Hwan Lee, Mi-Hyun Ahn, Mira Song, Kyung-Jin Lee, Doo-Byoung Oh, Youngkwan Kim, Kisung Ko
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A192522

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원문정보

초록

영어
The mAb CO17-1A recognizes the colorectal antigen GA733-2E, which highly is expressed on the surface membrane of human colorectal carcinoma cells. The higher yield of CO17-1A mAb reflect the use of the ER retrieval motif KDEL(Lys-Asp-Glu-Leu), In this study, the expression level of mAb CO17-1A was compared between mAbs fused with and without KDEL in plant. Western blot showed that the expression levels of mAb CO17-1A with KDEL were significantly higher than that without KDEL Cell ELISA with human colorectal carcinoma cell line confirmed that expression of the mAb with KDEL was significantly higher than that of mAb without KDEL. Flow cytometry analysis showed that the Fc domains of both purified mAbP (with and without KDEL) and mammalian-derived mAb have similar binding activity to the FcγRI receptor. The glycosylation analysis revealed that mAb CO17-1A without KDEL had plant specific glycans structure while mAb CO17-1A with KDEL had mainly oligomannose type glycans. Taken together, these studies suggest that KDEL affects expression of mAb and its glycan structures.

저자

  • Yangkang So [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Seul-Ki Lee [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Da-Young Park [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Zhe Lu [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Eun-Yi Oh [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Kwang-Wook Min [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Jung-Hwan Lee [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Mi-Hyun Ahn [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Mira Song [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Kyung-Jin Lee [ Korean Research Institute of Bioscience & Biotechnology, Daejeon 305-806, Korea ]
  • Doo-Byoung Oh [ Korean Research Institute of Bioscience & Biotechnology, Daejeon 305-806, Korea ]
  • Youngkwan Kim [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Kisung Ko [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국당과학회 [Korean Society for Glycoscience]
  • 설립연도
    2006
  • 분야
    의약학>약학
  • 소개
    본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.

간행물

  • 간행물명
    한국당과학회 학술대회
  • 간기
    연간
  • 수록기간
    2006~2022
  • 십진분류
    KDC 517 DDC 614

이 권호 내 다른 논문 / 한국당과학회 학술대회 4th 2009 Annual Meeting of Korean Society for Glycoscience in 2009

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