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Effect of ER signal peptides (KDEL) on expression and function of anti-virus monoclonal antibody in plant

첫 페이지 보기
  • 발행기관
    한국당과학회 바로가기
  • 간행물
    한국당과학회 학술대회 바로가기
  • 통권
    4th 2009 Annual Meeting of Korean Society for Glycoscience in 2009 (2009.11)바로가기
  • 페이지
    pp.45-45
  • 저자
    Da-Young Park, Yangkang So, Kyung-Jin Lee, Zhe Lu, Eun-Yi Oh, Kwang-Wok Min, Seul-Ki Lee, Jung-Hwan Lee, Mi-Hyun Ahn, Mira Song, Doo-Byoung Oh, Youngkwan Kim, Kisung Ko
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A192520

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원문정보

초록

영어
Plant genetic engineering has led to the production of plant-derived mAb (mAbP), which provides a safe and economically feasible alternative to the current antibody expression systems. In this study, the expression levels of mAbP SO57 with or without ER-signal peptides (Lys-Asp-Gly-Leu;KDEL) in transgenic tobacco plants were analysed in transgenic plant. PCR and Reverse Transcription-PCR analyses showed existence of heavy and light chain genes of mAb with or without KEDL and their transcription in plant, respectively. Western blot showed that the expression levels of mAbP SO57 with KDEL were significantly higher than that without KDEL. Flow cytometry analysis showed that the Fc domains of both purified mAbP and mammalian-derived mAb have similar binding activity to the FcγRI receptor. High-performance liquid chromatography analysis showed that the mAbP SO57 with KDEL had glycan profile with both oligomannose and golgi type, whereas the mAbP SO57 without KDEL had only golgi type glycans. Neutralizing analysis with rabies virus CVS-11 showed the similar neutralizing activity between mAbP SO57 with and without KDEL. These results suggest that the potential of mAbP SO57 for rabies immunotherapy is regardless of plant specific glycan structures.

저자

  • Da-Young Park [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Yangkang So [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Kyung-Jin Lee [ Korean Research Institute of Bioscience & Biotechnology, Daejeon ]
  • Zhe Lu [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Eun-Yi Oh [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Kwang-Wok Min [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Seul-Ki Lee [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Jung-Hwan Lee [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Mi-Hyun Ahn [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Mira Song [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Doo-Byoung Oh [ Korean Research Institute of Bioscience & Biotechnology, Daejeon ]
  • Youngkwan Kim [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]
  • Kisung Ko [ Department of Biological Science, College of Natural Sciences, Wonkwang University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국당과학회 [Korean Society for Glycoscience]
  • 설립연도
    2006
  • 분야
    의약학>약학
  • 소개
    본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.

간행물

  • 간행물명
    한국당과학회 학술대회
  • 간기
    연간
  • 수록기간
    2006~2022
  • 십진분류
    KDC 517 DDC 614

이 권호 내 다른 논문 / 한국당과학회 학술대회 4th 2009 Annual Meeting of Korean Society for Glycoscience in 2009

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