Earticle

초록

영어

Cancer is one of serious human diseases that causes angiogenesis, metastasis, and abnormal cell divisions including genetic change. Thus, lots of researchers have been investigated to inhibit cancer. Human 90K (h90K) protein has very crucial role for inhibition of cancer metastasis. h90K is a glycoprotein having potential 7 N-glycosylation sites (4 sites are clear but remaining 3 sites are not yet). In this work, we performed expression of recombinant h90K glycoprotein in insect Drosophila S2 cell system. We constructed stably-transfected S2-h90K cells by antibiotic selection and confirmed h90K expression using Western Blot and in vitro bioactivity analyses. We also identified N-glycan patterns of recombinant h90K glycoprotein using HPLC and MALDI-TOF MS. Based on the identified N-glycan structures, we next engineered N-glycosylation pathway to develop advanced N-glycan patterns using multiple expression platform strategy with recombinant baculoviruses. We found that infection with recombinant baculovirus having galactosyltransferase resulted advanced bi-antennary galactosyl form of N-glycans in h90K glycoprotein.

저자

• Kyoung Ro Kim [ National Research Laboratory of Molecular Biotechnology, Dept. of Chemical Engineering, POSTECH, Pohang, 790-784, Korea ]
• Dong Gyun Kang [ National Research Laboratory of Molecular Biotechnology, Dept. of Chemical Engineering, POSTECH, Pohang, 790-784, Korea ]
• Hyung Joon Cha [ National Research Laboratory of Molecular Biotechnology, Dept. of Chemical Engineering, POSTECH, Pohang, 790-784, Korea ]

참고문헌

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간행물

표지보기 관심저널 등록

• 간행물명

  한국당과학회 학술대회

• 간기

  연간

• 수록기간

  2006~2022

• 십진분류

  KDC 517 DDC 614