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SYM-12, Session III: Glycobiology II, Chair: Young-Kug Choo (Wonkwang Univ.)
Membrane Microdomains as a Platform of Carbohydrate-mediated Cell-cell Interactions during Gastrulation
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- 발행기관
- 한국당과학회 바로가기
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- 간행물
- 한국당과학회 학술대회 바로가기
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- 통권
- 4th 2009 Annual Meeting of Korean Society for Glycoscience in 2009 (2009.11)바로가기
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- 페이지
- pp.27-28
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- 저자
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- 언어
- 영어(ENG)
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- URL
- https://www.earticle.net/Article/A192507
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
- 영어
- It has been shown that membrane microdomains (or rafts) are important as a hot spot for signal transduction. However, little attention has been paid to the fact that glycan chains are enriched in the microdomains. We have recently proposed a hypothesis that the microdomains are involved in the protein- and glycan-mediated cell adhesion and the subsequent signal transduction, and have demonstrated it in sperm-egg interactions at fertilization of sea urchin [1-3] To extend this hypothesis in early embryogenesis, we have studied on microdomains in developing embryos of medaka fish, Oryziaslatipes. Here wes how several lines of evidence supporting our hypothesis: (i) The micro domains prepared from early gastrula embryos were rich in cholesterol and sphingomyelin, and contained E-cadherin and LeX-glycan-containing glycoproteins (LeX-gp) and glycolipids (LeX-gsl), together with transducer proteins like c-Srckinase. The LeX-glycans and E-cadherin are known to be involved in morula compaction (tight cell adhesion) in mouse; (ii) Disruption of the microdomains of early gastrula embryos by treatment with methyl-b-cyclodextrin (MBCD) or C2-ceramide, impaired epiboly, the first cell migration event that occurs during gastrulation process. The most typical phenotype of the microdomain-disrupted embryo was detachment of blastodermal cells. Interestingly, the MBCD-induced disruption of microdomains and the epiboly impairment were restored when cholesterol was added back to the embryos. Thus, microdomain formation is critical in epiboly [4]; (iii) The isolated microdomains could bind to the microdomains in the presence of Ca2+ in LeX-glycan- and E-cadherin-dependent manners. These results suggest that the microdomain formation is important in the protein- and carbohydrate-mediated cell-cell interactions.
저자
간행물 정보
발행기관
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- 발행기관명
- 한국당과학회 [Korean Society for Glycoscience]
- 설립연도
- 2006
- 분야
- 의약학>약학
- 소개
- 본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.
간행물
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- 간행물명
- 한국당과학회 학술대회
- 간기
- 연간
- 수록기간
- 2006~2022
- 십진분류
- KDC 517 DDC 614
이 권호 내 다른 논문 / 한국당과학회 학술대회 4th 2009 Annual Meeting of Korean Society for Glycoscience in 2009
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