SYM-7, Session II: Industrial Carbohydrate, Chair: Yong Il Park (Catholic Univ. of Korea)
Isolation and characterization of sodium orthovanadate (SOV) -induced water-soluble exo-polysaccharides in the photosynthetic green alga Haematococcuslacustris
4th 2009 Annual Meeting of Korean Society for Glycoscience in 2009 (2009.11)바로가기
페이지
pp.17-18
저자
Jae-Kweon Park, Choul-Gyun Lee, Sung-Min Kim, Yong-Il Park
언어
영어(ENG)
URL
https://www.earticle.net/Article/A192502
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원문정보
초록
영어
The aim of the present study was to analyze the influence of oxidative stress using sodium orthovanadate (SOV) on growth and production of water-soluble exo-polysaccharides in the photosynthetic green alga Haematococcus lacustris. Fourier Transform Infrared (FT-IR) analysis and elemental analysis were performed to determine the primary chemical structure of the polysaccharide, and its monosaccharide composition was characterized by a High Performance Anion-Exchange Chromatography (HPAEC) using Bio-LC (DX-500 Chromatography System, Dionex Co., USA). The molecular weight of the compound was determined by size-fractionation HPLC. Higher production of extracellular polysaccharide from H. lacustris following treatment of SOV was observed at day 2 as compared to untreated controls. Consequently, the total content of crude carbohydrates produced from SOV treated cells (51.2 mg/ml) was approximately 1.7- fold higher than that of the control cells (31.1 mg /ml) at day 2. The molecular mass of the major component of the purified water-soluble polysaccharide was estimated to be approximately 135-kDa by size-fractionation HPLC. In addition, Fourier Transform Infrared (FT-IR) and elemental analysis demonstrated the presence of sulfate groups in this polysaccharide. Taken collectively, our data suggest that oxidative stress by SOV to the cells inhibits cell growth and promotes the expression of water-soluble sulfated polysaccharides from H. lacustris.
저자
Jae-Kweon Park [ Department of Biotechnology, The Catholic University of Korea ]
Choul-Gyun Lee [ Institute of Industrial Biotechnology, Department of Biological Engineering, Inha University, Incheon ]
Sung-Min Kim [ Department of Biotechnology, The Catholic University of Korea ]
Yong-Il Park [ Department of Biotechnology, The Catholic University of Korea ]
Corresponding author.
본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.
간행물
간행물명
한국당과학회 학술대회
간기
연간
수록기간
2006~2022
십진분류
KDC 517DDC 614
이 권호 내 다른 논문 / 한국당과학회 학술대회 4th 2009 Annual Meeting of Korean Society for Glycoscience in 2009