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SYM-5, Session I: Carbohydrate Chemistry, Chair: Injae Shin (Yonsei Univ.)
Glycosylation of flavonoid in genetic engineered E. coli
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- 통권
- 4th 2009 Annual Meeting of Korean Society for Glycoscience in 2009 (2009.11)바로가기
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- 페이지
- pp.13-14
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- 저자
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- 언어
- 영어(ENG)
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- URL
- https://www.earticle.net/Article/A192499
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원문정보
초록
- 영어
- Flavonoids are a group of polyphenolic compounds that have been recognized as important due to their physiological and pharmacological roles and their health benefits. Glycosylation of flavonoids has a wide range of effects on flavonoid solubility, stability, and bioavailability. We previously generated the E. coli BL21 (DE3) Δpgi host by deleting the glucose-phosphate isomerase (Pgi) gene in E. coli BL21 (DE3). This host was further engineered for whole-cell biotransformation by integration of galU from E. coli K12, and expression of calS8 (UDP-glucose dehydrogenase) and calS9 (UDP-glucuronic acid decarboxylase) from Micromonospora echinospora spp. calichensis and arGt-4 (7-O-glycosyltransferase) from Arabidopsis thaliana to form E. coli (US89Gt-4), which is expected to produce glycosylated flavonoids. To test the designed system, the engineered host was fed naringenin as a substrate, and naringenin 7-O-xyloside, a glycosylated naringenin product, was detected. Product formation was verified by HPLC-LC/MS and ESI-MS/MS analyses. The reconstructed host can be applied for the production of various classes of glycosylated flavonoids. A whole-cell biotransformation system was designed in E. coli BL21 (DE3) Δpgi by integrating galU (glucose-1-phosphate uridylyltransferase) from E. coli K12, expressing calS8 (UDP-D-glucose dehydrogenase) and calS9 (UDP-D-glucuronic acid decarboxylase) from Micromonospora echinospora spp. calichensis and arGt-3 (3-O-glycosyltransferase) from Arabidopsis thaliana to form an engineered host, E. coli (US89Gt-3), for the production of glycosylated flavonoids when exogenously fed with flavonoids. To verify the system, quercetin was fed and a glycosylated product, quercetin 3-O-glucuronide, was produced but unable to produce quercetin 3-O-xyloside.
저자
간행물 정보
발행기관
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- 발행기관명
- 한국당과학회 [Korean Society for Glycoscience]
- 설립연도
- 2006
- 분야
- 의약학>약학
- 소개
- 본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.
간행물
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- 한국당과학회 학술대회
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