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Gangliosides are Biomarker Useful for Distinguishing Neuronal Cells Differentiated from Human Mesenchymal Stem Cells

첫 페이지 보기
  • 발행기관
    한국당과학회 바로가기
  • 간행물
    한국당과학회 학술대회 바로가기
  • 통권
    2009 한국 당과학회 하계 학술대회 (2009.06)바로가기
  • 페이지
    pp.67-68
  • 저자
    Eun-Jeong Jeong, Jae-Sung Ryu, Jung-Woo Jin, Hyo-Jung Yang, Chin-Hyoung Cho, So-Dam Lee, So-Hyun Lee, Young-Choo Lee, Young-Kug Choo
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A192454

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원문정보

초록

영어
Recent studies demonstrated that human mesenchymal stem cells (hMSCs) share molecular and cellular characteristics with bone marrow-derived mesenchymal stem cells (MSCs). hMSCs have the potential to differentiate into neural cells. Gangliosides are complex glycophingolipids with one or more sialic acids, the major components of cytoplasmic cell membranes. Numerous studies confirmed that the types of gangliosides and their expression levels are developmentally controlled and cell type-specific. Our recent studies proposed that expression of gangliosides is closely related to neural differentiation of embryonic stem cells in vitro. In this study, we investigated whether hMSCs are capable of differentiating into neural cells, and we analyzed the role of gangliosides in the neuronal differentiation of hMSCs. FACs analysis showed that the established cells have MSC characteristics. The cells did expressed MSC-specific surface antigene (CD44 and CD105), but not the hematopoietic markers CD45 and CD117. Next, hMSCs were differentiated into neuronal cell with induction media for 30h. Neuronal induced cells maintenance with differentiation media for 2weeks. High-performance thin-layer chromatography (HPTLC) showed that gangliosides GM3, GD3 and GD1a expressed in differentiated into neuronal cells for 2weeks, especially GD3 increased comparison to control cell. Immunofluorescence staining also agreed with the results of HPTLC analysis. These differentially expressed gangliosides suggest that gangliosides may have specific functions in stem cell and during neuronal differentiation. Therefore, thess results also suggest that regulation of gangliosides expression have used as differentiation marker of neuronal cell from hMSCs.

저자

  • Eun-Jeong Jeong [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan ]
  • Jae-Sung Ryu [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan ]
  • Jung-Woo Jin [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan ]
  • Hyo-Jung Yang [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan ]
  • Chin-Hyoung Cho [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan ]
  • So-Dam Lee [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan ]
  • So-Hyun Lee [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan ]
  • Young-Choo Lee [ Department of Biotechnology, Dong-A University, Busan, 604-714, Korea. ]
  • Young-Kug Choo [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국당과학회 [Korean Society for Glycoscience]
  • 설립연도
    2006
  • 분야
    의약학>약학
  • 소개
    본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.

간행물

  • 간행물명
    한국당과학회 학술대회
  • 간기
    연간
  • 수록기간
    2006~2022
  • 십진분류
    KDC 517 DDC 614

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