Ganglioside GQ1b has important functions in neuronal differentiation and neuronal development processs. The regulation of ganglioside levels is relationship to the induction of neuronal cell differentiation. In the previous study, we showed that expression of GT1b and GQ1b gangliosides in undifferentiated mouse embryonic stem cell were exhibit during their neural differentiation. We evaluated the co-localization of ganglioside GQ1b and neuroanl markers in an in vitor model of neuronal differentiation in mouse embryonic stem and found that neuronal differentiation was facilitated by the presence of increased gangliosides, especially GQ1b, due to daunorubicin. Furthermore, we investigated that important effect of GQ1b by overexpression and konckdown GQ1b in the neural differentiate. First, we designed a primer of a2,8-sialyltransferase (ST8Sia V) for producing GQ1b. In this study, we inserted ST8Sia V gene to overexpression (pEGFP-C2), and knock-down (pLKO.1 puro ShRNA) vectors. We will confirm the efficient transfectionof mouse embryonic stem cell by green fluorescent protein (GFP) and expressin of ganglioside GQ1b by immunofluorescence staining analyses.
저자
Jung-Woo Jin [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan ]
Jae-Sung Ryu [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan ]
Hyo-Jung Yang [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan ]
Jin-Hyoung Cho [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan ]
Eun-Jeong Jeong [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan ]
So-Dam Lee [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan ]
So-Hyun Lee [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan ]
Young-Choon Lee [ Department of Biotechnology, Dong-A University, Busan, 604-714, Korea. ]
Young-Kug Choo [ Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan ]
본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.