Jungdon Bae, Dooil Kim, miri Park, Jung Eun Park, Bo-Hyun Park, Dae-Sil Lee
언어
영어(ENG)
URL
https://www.earticle.net/Article/A192444
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원문정보
초록
영어
Thermus caldophilus GK24 (TCA) is an extremothermophile growing at 70℃ ,providing industrially important enzymes. From TCA genome sequence, all of gene sresponsible for carbohydrate biosynthetic network were identified .Among them, 120 enzyme genes have been expressed in E. coli and their reactions were characterized. In this lecture, Thermus-derived Carbohydrate Biosynthetic Network’ will be introduced, particularly focused on in vitro operation of a-glucan network’, glycolysis and pentose-phosphate pathway. 1) ‘a-Glucan Network’ showed to be the practical synthetic methods of various sugars such as trehalose, fructose, phosphosugars, sugar-nucleotides, a-glucan and so on. 2) in vitro Glycolysis using glycolytic enzymes have been operated for synthesizing pyruvate from glucose or glucose-1-phosphate. Accordingly, we could have managed to operate the glycolysis in flask. 3) And one pot reaction containing a-glucan phosphorylase, phospho glucomutase, glucose-6-phosphate isomerase, transaldolase and transketolase could synthesize sedoheptulose-7-phosphate from starch. Overall, ‘in vitro Cell Factory’ utilizing thermostable carbohydrate enzymes turned out to be a practical synthetic model to produce C3-C9 sugars, phosphosugars, sugar-nucleotides and biopolymers.
저자
Jungdon Bae [ Glycobiology Laboratory, Korea Research Institute of Bioscience and Biotechnology, Daejeon ]
Dooil Kim [ Glycobiology Laboratory, Korea Research Institute of Bioscience and Biotechnology, Daejeon ]
miri Park [ Glycobiology Laboratory, Korea Research Institute of Bioscience and Biotechnology, Daejeon ]
Jung Eun Park [ Glycobiology Laboratory, Korea Research Institute of Bioscience and Biotechnology, Daejeon ]
Bo-Hyun Park [ Glycobiology Laboratory, Korea Research Institute of Bioscience and Biotechnology, Daejeon ]
Dae-Sil Lee [ Glycobiology Laboratory, Korea Research Institute of Bioscience and Biotechnology, Daejeon ]
본 학회는 화학, 생화학, 분자생물학, 미생물학, 식품공학, 의학, 약학, 유전공학 및 생물공학, 환경 및 기타 공업 등 전 분야의 탄수화물관련 이론과 기술을 연구 발전시키고 산학협동을 통해 이를 보급하여 국내 관련 산업의 발전 및 국민생활의 과학화에 기여하고자 하며, 이러한 목표와 비젼의 실현을 위해 회원들이 적극적인 참여와 활동을 전개하고자 한다.