Earticle

초록

영어

Fucoidan was purified from the sporophyll of Korean Undaria pinnatifida and low-molecular weight fuco-oligosaccharides (LMFOs), ranging from 305 to 3,749 Da, were obtained by enzymic digestion of the purified fucoidan by Sphingomonas paucimobilis PF-1 (KCTC 11130BP). The enzyme was partially purified through 3 steps, including ammonium sulfate fractionation and affinity column chromatography. The enzyme was purified at 2.9% (activity/activity) yield from the concentrated supernatant of the sonicated PF-1. SDS-PAGE analysis of the partially enzyme showed three main band (34, 80 and 100 kDa) but still not purified to homogeneity. However, Native electrophoresis gel shown in gave the two main band at 30 and 60 kDa. The results presented here suggest that the 60 kDa subunit forms 2 species, namely 80-, 100 kDa subunits. The enzyme activity was assayed at different pH ranging from 3.0 to 8.0. The optimal activity was absorbed at pH 5.6. The enzyme was active in the range of pH 5-6, but the activity decreased at above pH 7 and below pH 4. When assayed at pH 5.6, crude enzyme showed its optimal activity at 30 ℃, and then sharply decreased by 30 ℃. When the incubation temperature was increased to 37, 42 ℃, there was decreased about 40-50 % of original activity the retained under the same conditions. The intact fucoidan and its LMFOs were compared for their anticoagulanting activities. Intact fucoidan prolonged activated partial thromboplastin time (APTT) at concentrations of 5 and 10 ug/ml, which was about 2.3- and 4.2- fold of the control (clotting time 90 and 168.4s each). LMFOs showed at concentrations of 100 and 200 ug/ml, which was about 2.3- and 3.9- fold of the control. Intact fucoidan prolonged thrombin time (TT) at concentrations of 5 and 10 ug/ml, which was about 42 and 68.7 s, respectively. It was about 2.8- and 4.6- fold. LMFOs approximately 1.7- and 2.6-fold at 50 and 100 ug/ml compared to that of control (14.8 s), respectively. LMFOs did not show any affect on prothrombin time (PT) at different concentrations compared with control but intact fucoidan effect increased with increasing concentration (10, 20, 50, 100 μg).

저자

• Woo Jung Kim [ Dert. of Biotechnology and Biomaterials Engineering Research Center, The Catholic University of Korea ]
• Yean Kyoung Koo [ Natural Products Research Institute, College of Pharmacy, Seoul National University, Gwanak-gu, Seoul ]
• Andriy Synytsya [ Dept. of Carbohydrate Chemistry and Technology, Institute of Chemical Technology in Prague, Technicka 5, 166 28 Praha 6, Czech Republic ]
• Hye Sook Yun-Choi [ Natural Products Research Institute, College of Pharmacy, Seoul National University, Gwanak-gu, Seoul ]
• Sung Min Kim [ Dert. of Biotechnology and Biomaterials Engineering Research Center, The Catholic University of Korea ]
• Yong-Il Park [ Dert. of Biotechnology and Biomaterials Engineering Research Center, The Catholic University of Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국당과학회 학술대회

• 간기

  연간

• 수록기간

  2006~2022

• 십진분류

  KDC 517 DDC 614